TNAP2 Antibody - #AF0630

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製品: TNAP2 Antibody
カタログ: AF0630
タンパク質の説明: Rabbit polyclonal antibody to TNAP2
アプリケーション: WB IHC IF/ICC
Cited expt.: IF/ICC
反応性: Human, Mouse, Monkey
予測: Horse, Rabbit, Dog
分子量: 72kDa; 73kD(Calculated).
ユニプロット: Q03169
RRID: AB_2834244

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 100ul $280 在庫あり
 200ul $350 在庫あり

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MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Monkey
予測:
Horse(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
TNAP2 Antibody detects endogenous levels of total TNAP2.
RRID:
AB_2834244
引用形式: Affinity Biosciences Cat# AF0630, RRID:AB_2834244.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

B94; EXOC3L3; exocyst complex component 3-like 3; Primary response gene B94 protein; TNAP2_HUMAN; TNF alpha-induced protein 2; Tnfaip2; tumor necrosis factor alpha induced protein 2; Tumor necrosis factor alpha-induced protein 2; tumor necrosis factor, alpha-induced protein 2 antibody;

免疫原

免疫原:

A synthesized peptide derived from human TNAP2, corresponding to a region within N-terminal amino acids.

Uniprot:

Q03169(TNAP2_HUMAN) >>Visit HPA database.

遺伝子(ID):
TNFAIP2(7127)
タンパク質の説明:
This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. The expression of this gene was shown to be induced by retinoic acid in a cell line expressing a oncogenic version of the retinoic acid receptor alpha fusion protein, which suggested that this gene may be a retinoic acid target gene in acute promyelocytic leukemia.
タンパク質配列:
MSEASSEDLVPPLEAGAAPYREEEEAAKKKKEKKKKSKGLANVFCVFTKGKKKKGQPSSAEPEDAAGSRQGLDGPPPTVEELKAALERGQLEAARPLLALERELAAAAAAGGVSEEELVRRQSKVEALYELLRDQVLGVLRRPLEAPPERLRQALAVVAEQEREDRQAAAAGPGTSGLAATRPRRWLQLWRRGVAEAAEERMGQRPAAGAEVPESVFLHLGRTMKEDLEAVVERLKPLFPAEFGVVAAYAESYHQHFAAHLAAVAQFELCERDTYMLLLWVQNLYPNDIINSPKLVGELQGMGLGSLLPPRQIRLLEATFLSSEAANVRELMDRALELEARRWAEDVPPQRLDGHCHSELAIDIIQITSQAQAKAESITLDLGSQIKRVLLVELPAFLRSYQRAFNEFLERGKQLTNYRANVIANINNCLSFRMSMEQNWQVPQDTLSLLLGPLGELKSHGFDTLLQNLHEDLKPLFKRFTHTRWAAPVETLENIIATVDTRLPEFSELQGCFREELMEALHLHLVKEYIIQLSKGRLVLKTAEQQQQLAGYILANADTIQHFCTQHGSPATWLQPALPTLAEIIRLQDPSAIKIEVATYATCYPDFSKGHLSAILAIKGNLSNSEVKRIRSILDVSMGAQEPSRPLFSLIKVG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

May play a role as a mediator of inflammation and angiogenesis.

タンパク質ファミリー:

Belongs to the SEC6 family.

参考文献

1). Targeting TNFAIP2 with NIR-II CRISPR-Cas9 nanosystem to overcome cisplatin resistance in laryngeal cancer. NPJ precision oncology, 2025 (PubMed: 40731142) [IF=7.9]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 2: Application of red blood cell membrane-encapsulated optogenetic CRISPR-Cas9 nanosystem for TNFAIP2 gene editing. A The working mechanism of the APC-HSP-Cas9 system, utilizing near-infrared (NIR-II) light to activate the CRISPR-Cas9 editing process on the target DNA; B TNFAIP2 gene editing efficiency under different transfection conditions, assessed by T7E1 digestion method to evaluate the percentage of indels using different delivery systems (PEI, Lipo, and varying concentrations of APC@RBCs); C Wild-type (WT) and mutant TNFAIP2 gene sequences, with the mutant sequence highlighted in red indicating deletions, insertions, and substitutions introduced by gene editing; D Quantitative PCR results showing a significant decrease in TNFAIP2 mRNA levels in cells treated with APC@RBCs (+) and APC@RBCs (-), indicating effective gene knockout; E Immunofluorescence images displaying TNFAIP2 protein expression in control, APC@RBCs (+), and APC@RBCs (-) treated cells, with red signal representing TNFAIP2 protein and blue signal representing DAPI-stained nuclei, Bar = 25 μm; F Quantitative immunofluorescence results. APC@RBCs (-): Cells transfected with the red blood cell membrane-encapsulated APC-HSP-Cas9 complex without 1064 nm laser irradiation; APC@RBCs (+): Cells transfected with the red blood cell membrane-encapsulated APC-HSP-Cas9 complex followed by 1064 nm laser irradiation; *p 

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