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Control-antibodies are used to detect the specific loading control proteins.

The internal control protein is used to ensure the uniformity of protein loading, so as to determine the expression of target protein, and is an important ruler for quantitative analysis of western blotting (WB) and other experimental results. Usually, the protein selected as internal reference is stably expressed in sample cell lines or tissues with moderate expression (as far as possible, it is detected in the same linear range as the target protein). In selecting the appropriate control protein, the molecular weight of the protein (different from the target protein), subcellular distribution, and the type of experimental treatment may also be considered.

Common internal control proteins are listed in the following table. Click the name to search for corresponding antibodies:

kDa

Whole cell

Mitochondrial

Nuclear

Membrane

Cytoskeleton

Serum

125

Vinculin

 

 

 

 

 

110

 

 

 

ATPase

 

 

75

 

 

 

 

 

Transferrin

66

 

 

Lamin B1

 

 

 

60

 

HSP60

 

 

 

 

55

 

 

HDAC1

 

 

 

50

Alpha tubulin

 

 

 

Alpha tubulin

 

50

Beta tubulin

 

 

 

Beta tubulin

 

40

Beta actin

 

 

 

Beta actin

 

35

GAPDH

 

TBP

 

 

 

30

 

VDAC1/Porin

PCNA

 

 

 

25

Cyclophilin B

 

 

 

 

 

20

Cofilin

COX IV

 

 

Cofilin

 

15

 

 

Histone H3