製品: AK1 Antibody
カタログ: AF0356
タンパク質の説明: Rabbit polyclonal antibody to AK1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 21kDa; 22kD(Calculated).
ユニプロット: P00568
RRID: AB_2834208

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
AK1 Antibody detects endogenous levels of total AK1.
RRID:
AB_2834208
引用形式: Affinity Biosciences Cat# AF0356, RRID:AB_2834208.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Adenylate kinase 1; Adenylate kinase isoenzyme 1; Adenylate kinase soluble; AK 1; Ak1; ATP AMP transphosphorylase; ATP-AMP transphosphorylase 1; KAD1; KAD1_HUMAN; Myokinase;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
AK1 Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Belongs to the adenylate kinase family. Monomer. Note: This description may include information from UniProtKB.
タンパク質配列:
MEEKLKKTKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRSEVSSGSARGKKLSEIMEKGQLVPLETVLDMLRDAMVAKVNTSKGFLIDGYPREVQQGEEFERRIGQPTLLLYVDAGPETMTQRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDSVFSQVCTHLDALK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Horse
92
Xenopus
92
Zebrafish
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00568 基板として

Site PTM Type Enzyme
M1 Acetylation
S19 Phosphorylation
K21 Acetylation
C25 S-Nitrosylation
K27 Ubiquitination
K31 Ubiquitination
T35 Phosphorylation
S38 Phosphorylation
T71 Phosphorylation
K83 Ubiquitination
K88 Ubiquitination
R107 Methylation
T145 Phosphorylation
Y153 Phosphorylation
K166 Ubiquitination
S178 Phosphorylation
S181 Phosphorylation
C187 S-Nitrosylation

研究背景

機能:

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Monomer.

タンパク質ファミリー:

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.

Belongs to the adenylate kinase family. AK1 subfamily.

研究領域

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Thiamine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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