CBX6 Antibody - #AF0411

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製品: CBX6 Antibody
カタログ: AF0411
タンパク質の説明: Rabbit polyclonal antibody to CBX6
アプリケーション: WB IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Xenopus
分子量: 44kDa; 44kD(Calculated).
ユニプロット: O95503
RRID: AB_2834202

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
CBX6 Antibody detects endogenous levels of total CBX6.
RRID:
AB_2834202
引用形式: Affinity Biosciences Cat# AF0411, RRID:AB_2834202.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Cbx 6; Cbx6; CBX6_HUMAN; Chromobox homolog 6; Chromobox protein homolog 6;

免疫原

免疫原:

A synthesized peptide derived from human CBX6, corresponding to a region within N-terminal amino acids.

Uniprot:

O95503(CBX6_HUMAN) >>Visit HPA database.

遺伝子(ID):
CBX6(23466)
タンパク質の説明:
CBX6 Involved in maintaining the transcriptionally repressive state of genes. Modifies chromatin, rendering it heritably changed in its expressibility (Probable). Component of the chromatin-associated polycomb complex (PcG). Note: This description may include information from UniProtKB.
タンパク質配列:
MELSAVGERVFAAESIIKRRIRKGRIEYLVKWKGWAIKYSTWEPEENILDSRLIAAFEQKERERELYGPKKRGPKPKTFLLKARAQAEALRISDVHFSVKPSASASSPKLHSSAAVHRLKKDIRRCHRMSRRPLPRPDPQGGSPGLRPPISPFSETVRIINRKVKPREPKRNRIILNLKVIDKGAGGGGAGQGAGALARPKVPSRNRVIGKSKKFSESVLRTQIRHMKFGAFALYKPPPAPLVAPSPGKAEASAPGPGLLLAAPAAPYDARSSGSSGCPSPTPQSSDPDDTPPKLLPETVSPSAPSWREPEVLDLSLPPESAATSKRAPPEVTAAAGPAPPTAPEPAGASSEPEAGDWRPEMSPCSNVVVTDVTSNLLTVTIKEFCNPEDFEKVAAGVAGAAGGGGSIGASK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Xenopus
100
Chicken
67
Horse
0
Sheep
0
Dog
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Possibly contributes to the target selectivity of the PRC1 complex by binding specific regions of chromatin. Recruitment to chromatin might occur in an H3K27me3-independent fashion (By similarity). May have a PRC1-independent function in embryonic stem cells (By similarity).

細胞の位置付け:

Nucleus. Chromosome.
Note: Uniformely distributed in the nucleoplasm (PubMed:18927235). Localizes to the inactivated X chromosome in females (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

参考文献

1). Identification of the Prognostic Biomarkers CBX6 and CBX7 in Bladder Cancer. Diagnostics (Basel, Switzerland), 2023 (PubMed: 37189494) [IF=3.6]

Application: WB    Species: Human    Sample: BLCA cell and tissue

Figure 11. Validation of CBX family protein expression levels in BLCA cell and tissue samples. (A) The expression levels of CBX family proteins were detected by Western blotting in normal urothelial cell lines (SV-HUC-1) and BLCA cell lines (5637 and T24) (n = 3). (B–I) Quantitative results of (A). (J,K) Expression levels of CBX6, CD25, and CD86 in BLCA tumor tissue samples analyzed by immunohistochemistry (IHC). (L,M) Expression level of CBX7, KIT, and CD14 analyzed by IHC in BLCA tumor tissue samples. * p < 0.05; ** p < 0.01; *** p < 0.001.

Application: IF/ICC    Species: Human    Sample: BLCA cell and tissue

Figure 11. Validation of CBX family protein expression levels in BLCA cell and tissue samples. (A) The expression levels of CBX family proteins were detected by Western blotting in normal urothelial cell lines (SV-HUC-1) and BLCA cell lines (5637 and T24) (n = 3). (B–I) Quantitative results of (A). (J,K) Expression levels of CBX6, CD25, and CD86 in BLCA tumor tissue samples analyzed by immunohistochemistry (IHC). (L,M) Expression level of CBX7, KIT, and CD14 analyzed by IHC in BLCA tumor tissue samples. * p < 0.05; ** p < 0.01; *** p < 0.001.

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