Phospho-MAPKAPK5 (Thr182) Antibody - #AF0061
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0061, RRID:AB_2834104.
折りたたみ／展開
MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPK5_HUMAN; MAPKAP kinase 5; MAPKAPK-5; MAPKAPK5; Mitogen activated protein kinase activated protein kinase 5; p38-regulated/activated protein kinase; PRAK;
免疫原
- Q8IW41 MAPK5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSEESDMDKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCATHPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALRHCHLLNIAHRDLKPENLLFKDNSLDAPVKLCDFGFAKIDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRRKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLKPLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEKLRDVIAQCILPQAGKGENEDEKLNEVMQEAWKYNRECKLLRDTLQSFSWNGRGFTDKVDRLKLAEIVKQVIEEQTTSHESQ
PTMs - Q8IW41 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S93 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K123 | Ubiquitination | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| T182 | Phosphorylation | P53778 (MAPK12) , P28482 (MAPK1) , O15264 (MAPK13) , P31152 (MAPK4) , P45984 (MAPK9) , Q15759 (MAPK11) , Q16539 (MAPK14) , Q16659 (MAPK6) | Uniprot |
| T186 | Phosphorylation | Uniprot | |
| Y188 | Phosphorylation | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| T214 | Phosphorylation | Uniprot | |
| Y242 | Phosphorylation | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| T368 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | Uniprot | |
| S472 | Phosphorylation | Uniprot |
PTMs - Q8IW41 酵素として
| Substrate | Site | Source |
|---|---|---|
| O00418 (EEF2K) | S377 | Uniprot |
| O43524 (FOXO3) | S215 | Uniprot |
| O60229 (KALRN) | S487 | Uniprot |
| P04637 (TP53) | S37 | Uniprot |
| P04792 (HSPB1) | S15 | Uniprot |
| P04792 (HSPB1) | S78 | Uniprot |
| P04792 (HSPB1) | S82 | Uniprot |
| P07101-3 (TH) | S19 | Uniprot |
| P07101-3 (TH) | S40 | Uniprot |
| P07101-3 (TH) | S404 | Uniprot |
| P25685 (DNAJB1) | S149 | Uniprot |
| P25685 (DNAJB1) | S151 | Uniprot |
| P25685 (DNAJB1) | S171 | Uniprot |
| P31152 (MAPK4) | S186 | Uniprot |
| P47712 (PLA2G4A) | S727 | Uniprot |
| Q15382 (RHEB) | S130 | Uniprot |
| Q92599 (SEPT8) | S163 | Uniprot |
| Q92599 (SEPT8) | S192 | Uniprot |
研究背景
Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.
Phosphorylated on Thr-182 ERK3/MAPK6 or ERK4/MAPK4; which is the regulatory phosphorylation site and is located on the T-loop/loop 12, leading to activation. Phosphorylation at Thr-182 by p38-alpha/MAPK14, p38-beta/MAPK11 is subject to debate. Phosphorylated at Ser-115 by PKA/PRKACA, leading to localization to the cytoplasm. Autophosphorylated (By similarity).
Cytoplasm. Nucleus.
Note: Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export.
Expressed ubiquitously.
Interacts with ERK3/MAPK6 and ERK4/MAPK4 (via FRIEDE motif); the interaction is direct (By similarity). Interacts with YWHAE; the interaction prevents phosphorylation of HSP27/HSPB1 leading to disrupt F-actin polymerization. Interacts with SQSTM1.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
研究領域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
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