HAS2 Antibody - #BF0034

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製品: HAS2 Antibody
カタログ: BF0034
タンパク質の説明: Mouse monoclonal antibody to HAS2
アプリケーション: WB IHC IF/ICC ELISA
Cited expt.: WB, IF/ICC
反応性: Human
分子量: 63kDa(Observed); 64kD(Calculated).
ユニプロット: Q92819
RRID: AB_2834056

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MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Mouse
アプリケーション:
ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [AFB2034]
特異性:
HAS2 antibody detects endogenous levels of total HAS2.
RRID:
AB_2834056
引用形式: Affinity Biosciences Cat# BF0034, RRID:AB_2834056.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

HA synthase 2; has2; HAS2_HUMAN; Hyaluronan synthase 2; Hyaluronate synthase 2; Hyaluronic acid synthase 2;

免疫原

免疫原:

Purified recombinant fragment of human HAS2 expressed in E. Coli.

Uniprot:

Q92819(HYAS2_HUMAN) >>Visit HPA database.

遺伝子(ID):
HAS2(3037)
発現特異性:
Q92819 HYAS2_HUMAN:

Expressed in fibroblasts.

タンパク質の説明:
Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds.
タンパク質配列:
MHCERFLCILRIIGTTLFGVSLLLGITAAYIVGYQFIQTDNYYFSFGLYGAFLASHLIIQSLFAFLEHRKMKKSLETPIKLNKTVALCIAAYQEDPDYLRKCLQSVKRLTYPGIKVVMVIDGNSEDDLYMMDIFSEVMGRDKSATYIWKNNFHEKGPGETDESHKESSQHVTQLVLSNKSICIMQKWGGKREVMYTAFRALGRSVDYVQVCDSDTMLDPASSVEMVKVLEEDPMVGGVGGDVQILNKYDSWISFLSSVRYWMAFNIERACQSYFGCVQCISGPLGMYRNSLLHEFVEDWYNQEFMGNQCSFGDDRHLTNRVLSLGYATKYTARSKCLTETPIEYLRWLNQQTRWSKSYFREWLYNAMWFHKHHLWMTYEAIITGFFPFFLIATVIQLFYRGKIWNILLFLLTVQLVGLIKSSFASCLRGNIVMVFMSLYSVLYMSSLLPAKMFAIATINKAGWGTSGRKTIVVNFIGLIPVSVWFTILLGGVIFTIYKESKRPFSESKQTVLIVGTLLYACYWVMLLTLYVVLINKCGRRKKGQQYDMVLDV

研究背景

機能:

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction and it is particularly responsible for the synthesis of high molecular mass hyaluronan. Required for the transition of endocardial cushion cells into mesenchymal cells, a process crucial for heart development. May also play a role in vasculogenesis. High molecular mass hyaluronan also play a role in early contact inhibition a process which stops cell growth when cells come into contact with each other or the extracellular matrix (By similarity).

細胞の位置付け:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in fibroblasts.

タンパク質ファミリー:

Belongs to the NodC/HAS family.

参考文献

1). CIRBP Enhances the Function of Yak Cumulus Cells by Activating AMPK/mTOR-Mediated Mitophagy. Biomolecules, 2025 (PubMed: 40563401) [IF=5.5]

Application: WB    Species: Yak    Sample: Yak Cumulus Cells

Figure 3. Overexpression of CIRBP improves YCC function. (A) Steroid-synthesis-related factors expression level after CIRBP overexpression, n = 4. (B) The mRNA expression level of cumulus-diffusion-related factors, n = 4. (C) The secretion of E2 and P4, n = 4. (D) E2 and P4 synthesis-related protein bands and analysis, n = 3. (E) Cumulus-diffusion-related protein bands and analysis, n = 3. (F) Immunofluorescence detection of cumulus-diffusion-related proteins. Values are mean ± SE, *: p < 0.05, **: p < 0.01, NS: no difference. Original Western blot images are provided in the Supplementary Materials.

Application: IF/ICC    Species: Yak    Sample: Yak Cumulus Cells

Figure 3. Overexpression of CIRBP improves YCC function. (A) Steroid-synthesis-related factors expression level after CIRBP overexpression, n = 4. (B) The mRNA expression level of cumulus-diffusion-related factors, n = 4. (C) The secretion of E2 and P4, n = 4. (D) E2 and P4 synthesis-related protein bands and analysis, n = 3. (E) Cumulus-diffusion-related protein bands and analysis, n = 3. (F) Immunofluorescence detection of cumulus-diffusion-related proteins. Values are mean ± SE, *: p < 0.05, **: p < 0.01, NS: no difference. Original Western blot images are provided in the Supplementary Materials.
2). GRIM19 deficiency aggravates metabolic disorder and ovarian dysfunction in PCOS. Biochimica et biophysica acta. Molecular basis of disease, 2024 (PubMed: 38360073) [IF=4.2]

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