LGR5 Antibody - #DF2816

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製品: LGR5 Antibody
カタログ: DF2816
タンパク質の説明: Rabbit polyclonal antibody to LGR5
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human, Mouse
予測: Pig, Bovine, Sheep
分子量: 100 kDa; 100kD(Calculated).
ユニプロット: O75473
RRID: AB_2840022

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(82%), Bovine(82%), Sheep(82%)
クローナリティ:
Polyclonal
特異性:
LGR5 Antibody detects endogenous levels of total LGR5.
RRID:
AB_2840022
引用形式: Affinity Biosciences Cat# DF2816, RRID:AB_2840022.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FEX; G protein coupled receptor 49; G protein coupled receptor 67; g protein-coupled receptor fex; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; GPR 49; GPR 67; GPR49; GPR67; GRP 49; GRP49; HG 38; HG38; Leucine rich repeat containing G protein coupled receptor 5; Leucine-rich repeat-containing G-protein coupled receptor 5; LGR 5; LGR5; LGR5_HUMAN; MGC117008; Orphan G protein coupled receptor HG38;

免疫原

免疫原:

A synthesized peptide derived from human LGR5, corresponding to a region within the internal amino acids.

Uniprot:

O75473(LGR5_HUMAN) >>Visit HPA database.

遺伝子(ID):
LGR5(8549)
発現特異性:
O75473 LGR5_HUMAN:

Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.

タンパク質の説明:
Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.;
タンパク質配列:
MDTSRLGVLLSLPVLLQLATGGSSPRSGVLLRGCPTHCHCEPDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRHVPTEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTEIPVQAFRSLSALQAMTLALNKIHHIPDYAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELGFHSNNIRSIPEKAFVGNPSLITIHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLESLTLTGAQISSLPQTVCNQLPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIKVDTFQQLLSLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPITGLHGLTHLKLTGNHALQSLISSENFPELKVIEMPYAYQCCAFGVCENAYKISNQWNKGDNSSMDDLHKKDAGMFQAQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDGWLIRIGVWTIAVLALTCNALVTSTVFRSPLYISPIKLLIGVIAAVNMLTGVSSAVLAGVDAFTFGSFARHGAWWENGVGCHVIGFLSIFASESSVFLLTLAALERGFSVKYSAKFETKAPFSSLKVIILLCALLALTMAAVPLLGGSKYGASPLCLPLPFGEPSTMGYMVALILLNSLCFLMMTIAYTKLYCNLDKGDLENIWDCSMVKHIALLLFTNCILNCPVAFLSFSSLINLTFISPEVIKFILLVVVPLPACLNPLLYILFNPHFKEDLVSLRKQTYVWTRSKHPSLMSINSDDVEKQSCDSTQALVTFTSSSITYDLPPSSVPSPAYPVTESCHLSSVAFVPCL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
82
Bovine
82
Sheep
82
Rabbit
78
Chicken
61
Horse
56
Xenopus
56
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Golgi apparatus>trans-Golgi network membrane>Multi-pass membrane protein.
Note: Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans-Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (PubMed:23439653).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family.

参考文献

1). A Nucleophilicity-Engineered DNA Ligation Blockade Nanoradiosensitizer Induces Irreversible DNA Damage to Overcome Cancer Radioresistance. Advanced materials (Deerfield Beach, Fla.), 2024 (PubMed: 39246208) [IF=27.4]
2). Molecular glue triggers degradation of PHGDH by enhancing the interaction between DDB1 and PHGDH. Acta pharmaceutica Sinica. B, 2024 (PubMed: 39309493) [IF=14.7]

Application: WB    Species: Mouse    Sample:

Figure 5. The regulatory role of PHGDH in maintaining the stemness of CRC cells. (A) Western blot analysis of PHGDH protein level in HCT-116 cells with stable expression of scrambled or PHGDH shRNA, and in parallel or PHGDH overexpression HCT-15 cells. (B) Representative micrographs of tumorspheres from HCT-116 cells bearing scrambled or PHGDH shRNA (up) or PHGDH-overexpression HCT-15 cells (down). (C) Quantification of the numbers of tumorspheres in (B). (D) Limiting dilution analysis (LDA) to assess the sphere-initiation capacity of stable PHGDH knockdown or overexpression cells. Cells were planted into 96-well plates at densities of 8, 15, 31, or 62 cells per well and cultured for 10 days, followed by counting of wells containing tumorspheres and analysis utilizing ELDA software. (E) Representative images of colony formation in soft agar. (F) Quantification of colony numbers in (E). (G) Quantitative RT-PCR analysis of CRC stem cell markers (LGR5, CD133, CD44, and CD166). (H) Detection of PHGDH protein levels of CD44 and LGR5 by Western blot. (I) Limiting dilution tumorigenesis assays conducted by subcutaneously injecting scrambled or PHGDH shRNA cells (1 × 104, 1 × 105, and 1 × 106) into BALB/c nude mice. After 39 days, animals were sacrificed. The ratio of formed tumors to the total number in each group is presented in a table and illustrated in a pie chart. (J) Images of tumors excised from mice injected with scrambled or PHGDH shRNA cells. (K) Tumor tissue weight. (L) Tumor volume growth curve for each group. (M) Western blot analysis of CD133, CD44, LGR5 and CD166 level in sh-PHGDH group and NC group. Statistical significance was determined by unpaired t-test. Error bars denoted SEM, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.
3). A bioactive calcium silicate nanowire-containing hydrogel for organoid formation and functionalization. Materials horizons, 2024 (PubMed: 38586926) [IF=12.2]
4). Bazi Bushen capsule improves the deterioration of the intestinal barrier function by inhibiting NLRP3 inflammasome-mediated pyroptosis through microbiota-gut-brain axis. Frontiers in microbiology, 2024 (PubMed: 38260869) [IF=5.2]
5). Antimicrobial agent chloroxylenol targets β‑catenin‑mediated Wnt signaling and exerts anticancer activity in colorectal cancer. International Journal of Oncology, 2023 (PubMed: 37681484) [IF=4.5]
6). Hymenolepis nana antigens alleviate ulcerative colitis by promoting intestinal stem cell proliferation and differentiation via AhR/IL-22 signaling pathway. PLoS neglected tropical diseases, 2024 (PubMed: 39666730) [IF=3.4]
7). Aligned membranes regulate wound healing via MMP12 secreted by macrophages. PloS one, 2025 (PubMed: 39813228) [IF=2.9]
8). Organoid culture of mouse fallopian tube epithelial stem cells with a thermo-reversible gelation polymer. TISSUE & CELL, 2021 (PubMed: 34454367) [IF=2.7]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 4. Expression of the epithelial cell marker EpCAM and the stem cell marker LGR5 in the spheres. Both EpCAM (A-C) and LGR5 (D-F) were extensively expressed in the spheres. Ab (-), without application of the first antibody as a negative control (G-I). Scale bars = 25 μm.
9). Hydrogel Derived from Decellularized Porcine Small Intestinal Submucosa as a Physical Shielding Repaired the Gut Epithelial Barrier of Murine Ulcerative Colitis. Advanced Functional Materials, 2024

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