CHRM3 Antibody - #DF2781

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製品: CHRM3 Antibody
カタログ: DF2781
タンパク質の説明: Rabbit polyclonal antibody to CHRM3
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 85 kDa,; 66kD(Calculated).
ユニプロット: P20309
RRID: AB_2839987

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
クローナリティ:
Polyclonal
特異性:
CHRM3 Antibody detects endogenous levels of total CHRM3.
RRID:
AB_2839987
引用形式: Affinity Biosciences Cat# DF2781, RRID:AB_2839987.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AChR; ACM3_HUMAN; cholinergic receptor muscarinic 3; CHRM 3; CHRM3; EGBRS; HM 3; HM3; m3 muscarinic acetylcholine receptor; M3 muscarinic receptor; muscarinic 3; Muscarinic acetylcholine receptor M3; muscarinic cholinergic m3 receptor; muscarinic m3 receptor;

免疫原

免疫原:
Uniprot:

P20309(ACM3_HUMAN) >>Visit HPA database.

遺伝子(ID):
CHRM3(1131)
タンパク質の説明:
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.;
タンパク質配列:
MTLHNNSTTSPLFPNISSSWIHSPSDAGLPPGTVTHFGSYNVSRAAGNFSSPDGTTDDPLGGHTVWQVVFIAFLTGILALVTIIGNILVIVSFKVNKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLACDLWLAIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIYKETEKRTKELAGLQASGTEAETENFVHPTGSSRSCSSYELQQQSMKRSNRRKYGRCHFWFTTKSWKPSSEQMDQDHSSSDSWNNNDAAASLENSASSDEEDIGSETRAIYSIVLKLPGHSTILNSTKLPSSDNLQVPEEELGMVDLERKADKLQAQKSVDDGGSFPKSFSKLPIQLESAVDTAKTSDVNSSVGKSTATLPLSFKEATLAKRFALKTRSQITKRKRMSLVKEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTFWNLGYWLCYINSTVNPVCYALCNKTFRTTFKMLLLCQCDKKKRRKQQYQQRQSVIFHKRAPEQAL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
83
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20309 基板として

Site PTM Type Enzyme
S291 Phosphorylation
Y365 Phosphorylation
S366 Phosphorylation
S413 Phosphorylation
S457 Phosphorylation
S578 Phosphorylation

研究背景

機能:

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Basolateral cell membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein.
Note: Colocalizes with TMEM147 in the endoplasmic reticulum (ER) membrane. TMEM147 impairs its trafficking to the cell membrane leading to its retention in the ER membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homodimer; the dimers can form tetramers. Interacts with NALCN (By similarity). Interacts with TMEM147.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.

研究領域

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Sensory system > Taste transduction.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

参考文献

1). Porcine circovirus-like virus P1 activates pancreatic secretion pathway by interacting with CHRM3 protein. VETERINARY MICROBIOLOGY (PubMed: 35810687) [IF=3.3]

Application: WB    Species: piglets    Sample: 3D4 cells

Fig. 5. Interference with CHRM3 protein inhibited the activation of the pathway caused by P1 virus. (A)Schematic diagram of shRNA and sequencing results. Red and green boxes indicate the interference sites of shRNA-1. (B)Real-time PCR analysis of CHRM3 mRNA expression in 3D4 cells transfected with shRNA-N (empty vector control), shRNA-1, or shRNA-2 at 24 h and 48 h post-transfection (n = 3). Immunoblot analysis of CHRM3 protein in CHRM3 knockdown 3D4 cells. The right panel is the intensity of the CHRM3 protein level normalized to that of β-actin (n = 3). The 3D4 cells were transfected with shRNA-N or shRNA-1 for 48 h and then infected with P1 (shRNA-1 +P1) or uninfected (shRNA-N or shRNA-1). Real-time PCR (D) and Western blot (E) were used to detect the expression of the main components of the pancreatic secretory pathway (CHRM3, Gq, PLC-β2, PRKCA, Rab3D, RhoA) at 48 h post-infection (n = 3). The gray value of the Western blot band (F) was analyzed by ImageJ software. Student's t-test was used to examine the differences between groups (∗P 

Application: IF/ICC    Species: Human    Sample: HEK293 cells

Fig. 3. Both the VP1 protein of the virus and the CHRM3 protein upregulate main components of the pancreatic secretory pathway. (A) Amplification of VP1 and CHRM3 genes and restriction digestion identification of recombinant plasmids. Western blot (B) and Immunofluorescence (C) analysis of the expression of VP1 and CHRM3 protein in HEK293 cells transfected with pcDNA3.1-HA-CHRM3 or pcDNA3.1-VP1-Flag at 48 h post-transfection. (D) Western blot analysis of the expression of VP1 and CHRM3 proteins in 3D4 cells transfected with pcDNA3.1-HA-CHRM3 or pcDNA3.1-VP1-Flag at 24 h, 48 h, and 72 h post-transfection. The right panel is the intensity of the CHRM3 protein level normalized to that of β-actin (n = 3). (E) Real-time PCR analysis of CHRM3, Gq, PLC-β2, PRKCA, Rab3D, RhoA, Rac1, and amyA mRNA expression levels in 3D4 cells transfected with pcDNA3.1-VP1-Flag, pcDNA3.1-HA-CHRM3, or pcDNA 3.1 vector (control) at 24 h, 48 h, or 72 h post-transfection (n = 3). The differences between groups were analyzed by Student's t-test (∗P 

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