GPR84 Antibody - #DF2769

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製品: GPR84 Antibody
カタログ: DF2769
タンパク質の説明: Rabbit polyclonal antibody to GPR84
アプリケーション: WB IHC
Cited expt.: IHC
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 38 kDa(Observed); 44kD(Calculated).
ユニプロット: Q9NQS5
RRID: AB_2839975

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#DF2769-BP

GPR84 Peptide

1mg
関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(89%), Rabbit(89%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
GPR84 Antibody detects endogenous levels of total GPR84.
RRID:
AB_2839975
引用形式: Affinity Biosciences Cat# DF2769, RRID:AB_2839975.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

EX 33; EX33; G protein coupled receptor 84; G protein coupled receptor EX 33; G protein coupled receptor EX33; G-protein coupled receptor 84; GPCR 4; GPCR4; GPR 84; GPR84; GPR84_HUMAN; Inflammation related G protein coupled receptor EX 33; Inflammation related G protein coupled receptor EX33; Inflammation-related G-protein coupled receptor EX33;

免疫原

免疫原:

A synthesized peptide derived from human GPR84, corresponding to a region within N-terminal amino acids.

Uniprot:

Q9NQS5(GPR84_HUMAN) >>Visit HPA database.

遺伝子(ID):
GPR84(53831)
発現特異性:
Q9NQS5 GPR84_HUMAN:

Expressed predominantly in hematopoietic tissues. High levels detected in the bone marrow and lower levels in the peripheral leukocytes and lung. Also expressed in brain, heart, muscle, colon, thymus, spleen, kidney, liver, placenta and intestine. Within the leukocyte population expression is higher in neutrophils and eosinophils relative to T- or B-lymphocytes.

タンパク質の説明:
Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.
タンパク質配列:
MWNSSDANFSCYHESVLGYRYVAVSWGVVVAVTGTVGNVLTLLALAIQPKLRTRFNLLIANLTLADLLYCTLLQPFSVDTYLHLHWRTGATFCRVFGLLLFASNSVSILTLCLIALGRYLLIAHPKLFPQVFSAKGIVLALVSTWVVGVASFAPLWPIYILVPVVCTCSFDRIRGRPYTTILMGIYFVLGLSSVGIFYCLIHRQVKRAAQALDQYKLRQASIHSNHVARTDEAMPGRFQELDSRLASGGPSEGISSEPVSAATTQTLEGDSSEVGDQINSKRAKQMAEKSPPEASAKAQPIKGARRAPDSSSEFGKVTRMCFAVFLCFALSYIPFLLLNILDARVQAPRVVHMLAANLTWLNGCINPVLYAAMNRQFRQAYGSILKRGPRSFHRLH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Sheep
89
Rabbit
89
Xenopus
67
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed predominantly in hematopoietic tissues. High levels detected in the bone marrow and lower levels in the peripheral leukocytes and lung. Also expressed in brain, heart, muscle, colon, thymus, spleen, kidney, liver, placenta and intestine. Within the leukocyte population expression is higher in neutrophils and eosinophils relative to T- or B-lymphocytes.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family.

参考文献

1). Novel macrophage-related gene prognostic index for glioblastoma associated with M2 macrophages and T cell dysfunction. Frontiers in Immunology, 2022 (PubMed: 36177003) [IF=5.7]

Application: IHC    Species: Human    Sample:

Figure 2 Prognostic significance of MRGPI-based groups. (A) Multivariate Cox regression analysis of 13 differentially expressed macrophage-related hub genes. (B) Cox regression analysis of the MRGPI and clinicopathological parameters based on the TCGA cohort. Covariates including age, gender, IDH mutation, ATRX mutation, MGMT promoter methylation status, TERT promoter mutation, and MRGPI were included in the initial univariate Cox regression. Covariates with p-values less than 0.01 were further included in the multivariate Cox model. (C, D) K-M analysis of the survival and tumor progression-free interval differences between MRGPI-based groups based on TCGA, CGGA325, and Gravendeel GBM cohorts. (E, F) Immunohistochemical staining of five genes at the protein level. The tissue was divided into core of the tumor (Tumor) and the margin containing infiltrating tumor cells (Peritumor) in three patients with a pathological diagnosis of GBM. The intensity of staining for the proteins encoded by these genes at the tissue level ranged from negative to positive, with E showing genes with significantly higher IHC scores in the tumor core. The IHC scores of the five genes were shown in F (Scale bar, 100μm). (G) Pan-cancer-based MRGPI prognostic significance. Samples were split into early (PFI < 6 months) and late (PFI > 12 months) relapse groups based on PFI. OV, Ovarian serous cystadenocarcinoma; LUAD, Lung adenocarcinoma; LUSC, Lung squamous cell carcinoma; PRAD, Prostate adenocarcinoma; BLCA, Bladder urothelial carcinoma; TGCT, Testicular germ cell tumors; ESCA, Esophageal carcinoma; PAAD, Pancreatic adenocarcinoma; LIHC, Liver hepatocellular carcinoma; KIRP, Kidney renal papillary cell carcinoma; SARC, Sarcoma; BRCA, Breast invasive carcinoma; MESO, Mesothelioma; COAD, Colon adenocarcinoma; STAD, Stomach adenocarcinoma; SKCM, Skin cutaneous melanoma; CHOL, Cholangiocarcinoma; KIRC, Kidney renal clear cell carcinoma; THCA, Thyroid carcinoma; UCEC, Uterine corpus endometrial carcinoma; CESC, Cervical squamous cell carcinoma and endocervical adenocarcinoma; HNSC, Head and neck squamous cell carcinoma; READ, Rectum adenocarcinoma; LGG, Lower grade glioma; KICH, Kidney chromophobe; UCS, Uterine carcinosarcoma; ACC, Adrenocortical carcinoma; PCPG, Pheochromocytoma and paraganglioma; UVM, Uveal melanoma. ***p < 0.001. ns, non significant.
2). Synthetic GPR84 Agonists in Colorectal Cancer: Effective in THP-1 Cells but Ineffective in BMDMs and MC38 Mouse Tumor Models. International journal of molecular sciences, 2025 (PubMed: 39859206) [IF=5.6]

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