GPR55 Antibody - #DF2752

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製品: GPR55 Antibody
カタログ: DF2752
タンパク質の説明: Rabbit polyclonal antibody to GPR55
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human
予測: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 37kDa(Observed); 37kD(Calculated).
ユニプロット: Q9Y2T6
RRID: AB_2839958

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#DF2752-BP

GPR55 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Bovine(80%), Horse(89%), Sheep(90%), Rabbit(90%), Dog(80%)
クローナリティ:
Polyclonal
特異性:
GPR55 Antibody detects endogenous levels of total GPR55.
RRID:
AB_2839958
引用形式: Affinity Biosciences Cat# DF2752, RRID:AB_2839958.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

G protein coupled receptor 55; G-protein coupled receptor 55; GPCR GPR55; GPR 55; Gpr55; GPR55_HUMAN; LPIR1; Probable G protein coupled receptor 55;

免疫原

免疫原:

A synthesized peptide derived from human GPR55, corresponding to a region within the internal amino acids.

Uniprot:

Q9Y2T6(GPR55_HUMAN) >>Visit HPA database.

遺伝子(ID):
GPR55(9290)
発現特異性:
Q9Y2T6 GPR55_HUMAN:

Expressed in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain or in the liver. Expressed in osteoclasts and osteoblasts.

タンパク質の説明:
May be involved in hyperalgesia associated with inflammatory and neuropathic pain (By similarity). Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca(2+) release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA. Putative cannabinoid receptor. May play a role in bone physiology by regulating osteoclast number and function.
タンパク質配列:
MSQQNTSGDCLFDGVNELMKTLQFAVHIPTFVLGLLLNLLAIHGFSTFLKNRWPDYAATSIYMINLAVFDLLLVLSLPFKMVLSQVQSPFPSLCTLVECLYFVSMYGSVFTICFISMDRFLAIRYPLLVSHLRSPRKIFGICCTIWVLVWTGSIPIYSFHGKVEKYMCFHNMSDDTWSAKVFFPLEVFGFLLPMGIMGFCCSRSIHILLGRRDHTQDWVQQKACIYSIAASLAVFVVSFLPVHLGFFLQFLVRNSFIVECRAKQSISFFLQLSMCFSNVNCCLDVFCYYFVIKEFRMNIRAHRPSRVQLVLQDTTISRG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
90
Sheep
90
Horse
89
Bovine
80
Dog
80
Chicken
67
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

May be involved in hyperalgesia associated with inflammatory and neuropathic pain (By similarity). Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca(2+) release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA. Putative cannabinoid receptor. May play a role in bone physiology by regulating osteoclast number and function.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain or in the liver. Expressed in osteoclasts and osteoblasts.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family.

参考文献

1). The GPR55 antagonist CID16020046 mitigates advanced glycation end products (AGEs)-induced chondrocyte activation. CHEMICO-BIOLOGICAL INTERACTIONS, 2020 (PubMed: 32360554) [IF=4.7]

Application: WB    Species: human    Sample: ATDC5 cells

Fig. 1. |Advanced Glycation End Products(AGEs) increased the expression of GPR55 in human ATDC5 cells. Cells were stimulated with 50, 100, and 200 μg/ml AGEs for 24 h. (A). Real time PCR analysis revealed that AGEs increased the expression of GPR55 at the mRNA levels; (B). Western blot analysis revealed that AGEs increased the expression of GPR55 at the protein levels (*, **, ***, P < 0.05, 0.01, 0.005 vs.previous group, n = 4).
2). A comprehensive survival and prognosis analysis of GPR55 expression in hepatocellular carcinoma. Aging, 2023 (PubMed: 37688769) [IF=3.9]

Application: WB    Species: human    Sample: HepG2 cells

Figure 9. (A) Protein levels of GPR55 were detected by western-blotting in HepG2 cells, respectively. (B) Protein levels of GPR55 were detected by western-blotting in 97H cells, respectively. (C) Determination of cell viability in HepG2 cells. (D) Determination of cell viability in 97H cells. (E) Transwell assay in HepG2 cells. (F) Transwell assay in 97H cells. (G) Immunofluorescence staining of GPR55 and PD-1. (H) Immunofluorescence staining of GPR55 and PD-L1. N = 3, ***P < 0.01, t-test.

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