TRIB2 Antibody - #DF2692

Figure 4 Target gene screening revealed that miR-100-5p directly targeted Trib2. (A) The target genes of miR-100-5p were predicted using miRDB, Targetsan, StarBase and miRcode. (B) Eight candidate target genes of miR-100-5p were obtained via Venn analysis. (C) The mRNA expression levels of proliferation marker gene Cdk4 and six candidate target genes were detected by qRT–PCR after miR-100-5p overexpression and inhibition at the proliferation stage in myoblasts. (D) The mRNA expression levels of the differentiation marker gene Myhc and six candidate target genes were detected by qRT–PCR in myoblasts after miR-100-5p overexpression and inhibition at the differentiation stage. (E,F) Wild-type binding sites (WT) and mutation binding sites (MUT) of miR-100-5p in the Mtmr3 3′UTR and Trib2 3′UTR regions. (G,H) In the dual-luciferase reporter assay, the pmirGLO–Mtmr3/Trib2–WT and pmirGLO–Mtmr3/Trib2–MUT report vectors were co–transfected with the miR-100-5p mimic or mimic NC into HEK293T cells. (I–K) Western-blot-detected protein expression of TRIB2 in myoblasts after transfection with miR-100-5p inhibitor and miR-100-5p mimic. The results are presented as the mean ± SEM (n = 3).