NUAK2 Antibody - #DF2671

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製品: NUAK2 Antibody
カタログ: DF2671
タンパク質の説明: Rabbit polyclonal antibody to NUAK2
アプリケーション: WB IHC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat
予測: Pig, Horse, Sheep, Rabbit, Dog
分子量: 80 kDa(Observed); 70kD(Calculated).
ユニプロット: Q9H093
RRID: AB_2839877

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#DF2671-BP

NUAK2 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(89%), Horse(100%), Sheep(89%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
NUAK2 Antibody detects endogenous levels of total NUAK2.
RRID:
AB_2839877
引用形式: Affinity Biosciences Cat# DF2671, RRID:AB_2839877.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DKFZp434J037; DKFZp686F01113; FLJ90349; NUAK family SNF1 like kinase 2; NUAK family SNF1-like kinase 2; Nuak2; NUAK2_HUMAN; Omphalocele kinase 2; SNARK; SNF1/AMP activated protein kinase; SNF1/AMP kinase related kinase; SNF1/AMP kinase-related kinase;

免疫原

免疫原:

A synthesized peptide derived from human NUAK2, corresponding to a region within the internal amino acids.

Uniprot:

Q9H093(NUAK2_HUMAN) >>Visit HPA database.

遺伝子(ID):
NUAK2(81788)
タンパク質の説明:
Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells.;
タンパク質配列:
MESLVFARRSGPTPSAAELARPLAEGLIKSPKPLMKKQAVKRHHHKHNLRHRYEFLETLGKGTYGKVKKARESSGRLVAIKSIRKDKIKDEQDLMHIRREIEIMSSLNHPHIIAIHEVFENSSKIVIVMEYASRGDLYDYISERQQLSEREARHFFRQIVSAVHYCHQNRVVHRDLKLENILLDANGNIKIADFGLSNLYHQGKFLQTFCGSPLYASPEIVNGKPYTGPEVDSWSLGVLLYILVHGTMPFDGHDHKILVKQISNGAYREPPKPSDACGLIRWLLMVNPTRRATLEDVASHWWVNWGYATRVGEQEAPHEGGHPGSDSARASMADWLRRSSRPLLENGAKVCSFFKQHAPGGGSTTPGLERQHSLKKSRKENDMAQSLHSDTADDTAHRPGKSNLKLPKGILKKKVSASAEGVQEDPPELSPIPASPGQAAPLLPKKGILKKPRQRESGYYSSPEPSESGELLDAGDVFVSGDPKEQKPPQASGLLLHRKGILKLNGKFSQTALELAAPTTFGSLDELAPPRPLARASRPSGAVSEDSILSSESFDQLDLPERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPLGDSCFSLTDCQEVTATYRQALRVCSKLT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
89
Sheep
89
Zebrafish
78
Chicken
78
Xenopus
67
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1.

PTMs:

Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Autophosphorylated in vitro.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

参考文献

1). Upregulation of NUAK2: A novel prognostic marker in breast cancer. HISTOLOGY AND HISTOPATHOLOGY, 2022 (PubMed: 36440828) [IF=2.5]

Application: WB    Species: Human    Sample: breast cancer tissues and cell

Fig. 1. Expression of NUAK2 is elevated in breast cancer tissues and cell lines. A. RT-qPCR was performed to determine the expression of NUAK2 in the breast cancer and normal tissues. B. Western blotting assays were performed to assess the protein levels of NUAK2 in 10 breast cancer tissues with paired adjacent non-tumoral tissues. C. Western blotting analysis of NUAK2 protein expression in breast cancer cell lines and primary cultured human mammary epithelial cell (HEMC).

Application: IHC    Species: Human    Sample: breast cancer tissues and cell

Fig. 2. Immunochemistry analyses of NUAK2 expression in breast cancer tissue samples. Representative images of negative staining of NUAK2 (A), weakly positive staining (+) of NUAK2 (B), positive staining (++) of NUAK2 (C) and strongly positive staining (+++) of NUAK2 (D). x 400.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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