PAX4 Antibody - #DF2605

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製品: PAX4 Antibody
カタログ: DF2605
タンパク質の説明: Rabbit polyclonal antibody to PAX4
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit
分子量: 42 kDa; 38kD(Calculated).
ユニプロット: O43316
RRID: AB_2839811

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(92%), Bovine(92%), Horse(92%), Sheep(83%), Rabbit(92%)
クローナリティ:
Polyclonal
特異性:
PAX4 Antibody detects endogenous levels of total PAX4.
RRID:
AB_2839811
引用形式: Affinity Biosciences Cat# DF2605, RRID:AB_2839811.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

KPD; MGC129960; MODY9; Paired box 4; Paired box gene 4; paired box homeotic gene 4; Paired box protein Pax-4; Paired domain gene 4; Pax4; PAX4_HUMAN;

免疫原

免疫原:
Uniprot:

O43316(PAX4_HUMAN) >>Visit HPA database.

遺伝子(ID):
PAX4(5078)
タンパク質の説明:
Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that binds to a common element in the glucagon, insulin and somatostatin promoters. Competes with PAX6 for this same promoter binding site. Isoform 2 appears to be a dominant negative form antagonizing PAX4 transcriptional activity.
タンパク質配列:
MHQDGISSMNQLGGLFVNGRPLPLDTRQQIVRLAVSGMRPCDISRILKVSNGCVSKILGRYYRTGVLEPKGIGGSKPRLATPPVVARIAQLKGECPALFAWEIQRQLCAEGLCTQDKTPSVSSINRVLRALQEDQGLPCTRLRSPAVLAPAVLTPHSGSETPRGTHPGTGHRNRTIFSPSQAEALEKEFQRGQYPDSVARGKLATATSLPEDTVRVWFSNRRAKWRRQEKLKWEMQLPGASQGLTVPRVAPGIISAQQSPGSVPTAALPALEPLGPSCYQLCWATAPERCLSDTPPKACLKPCWDCGSFLLPVIAPSCVDVAWPCLDASLAHHLIGGAGKATPTHFSHWP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Rabbit
92
Sheep
83
Zebrafish
58
Dog
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43316 基板として

Site PTM Type Enzyme
T26 Phosphorylation
T64 Phosphorylation

研究背景

機能:

Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that binds to a common element in the glucagon, insulin and somatostatin promoters. Competes with PAX6 for this same promoter binding site. Isoform 2 appears to be a dominant negative form antagonizing PAX4 transcriptional activity.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the paired homeobox family.

研究領域

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

参考文献

1). In utero exposure to phenanthrene induced islet cell dysfunction in adult mice: sex differences in the effects and potential causes. Science of The Total Environment (PubMed: 33513515) [IF=9.8]

Application: WB    Species: mice    Sample: islet cell

Fig. 4. The expression of pancreatic proteins that participate in islet cell function of adult F1 mice. The data were shown as the mean ± SE and analyzed with SPSS 19.0 software. Significant difference (p < 0.05) was tested using one-way ANOVA (Duncan's test), which are labeled by *. n = 4, 3 dams from each group, and 1–2 F1 mice from each dam.

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