ALKBH5 Antibody - #DF2585

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製品: ALKBH5 Antibody
カタログ: DF2585
タンパク質の説明: Rabbit polyclonal antibody to ALKBH5
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 44 kDa, 37 kDa(Observed); 44kD(Calculated).
ユニプロット: Q6P6C2
RRID: AB_2839791

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#DF2585-BP

ALKBH5 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
予測:
Pig(92%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%)
クローナリティ:
Polyclonal
特異性:
ALKBH5 Antibody detects endogenous levels of total ALKBH5.
RRID:
AB_2839791
引用形式: Affinity Biosciences Cat# DF2585, RRID:AB_2839791.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ABH5; AlkB, alkylation repair homolog 5 (E. coli); AlkB, alkylation repair homolog 5; Alkylated DNA repair protein alkB homolog 5; Alpha ketoglutarate dependent dioxygenase alkB homolog 5; OFOXD; OFOXD1; Oxoglutarate and iron-dependent oxygenase domain containing; Probable alpha ketoglutarate dependent dioxygenase ABH5; RNA demethylase ALKBH5;

免疫原

免疫原:

A synthesized peptide derived from human ALKBH5, corresponding to a region within the internal amino acids.

Uniprot:

Q6P6C2(ALKB5_HUMAN) >>Visit HPA database.

遺伝子(ID):
ALKBH5(54890)
発現特異性:
Q6P6C2 ALKB5_HUMAN:

Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.

タンパク質配列:
MAAASGYTDLREKLKSMTSRDNYKAGSREAAAAAAAAVAAAAAAAAAAEPYPVSGAKRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCSEAAGSPARKVKMRRH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
92
Chicken
82
Xenopus
63
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells (By similarity).

細胞の位置付け:

Nucleus speckle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.

タンパク質ファミリー:

Belongs to the alkB family.

参考文献

1). Pseudorabies virus exploits N6-methyladenosine modification to promote viral replication. Frontiers in Microbiology, 2023 (PubMed: 36819040) [IF=5.2]

Application: WB    Species: pig    Sample: PK15 cell

Figure 6. Demethylase FTO and ALKBH5 promoted PRV proliferation. (A) PK15 cells were transfected with the specified siRNAs (60 nM) for 24 h. FTO and ALKBH5 were assessed by immunoblotting analysis. β-actin was used as a loading control. (B) PK15 cells were transfected with the specified siRNAs and were mock transfected (MT) with transfection reagent alone for 24 h. PK15 cells were infected with PRV-FJ01 (MOI = 0.1) for 12 and 24 h. PRV DNA copies were evaluated by RT-qPCR analysis. (C) PK15 cells were transfected with the indicated siRNAs and were mock transfected (MT) with transfection reagent alone for 24 h. PK15 cells were infected with PRV-FJ01 (MOI = 0.1) for 24 h. PRV titers were assessed by TCID50 analysis. (D) PK15 cells were transfected with the indicated siRNAs and were mock transfected (MT) with transfection reagent alone for 24 h. PK15 cells were infected with PRV-FJ01 (MOI = 0.1) for 24 h. PRV gE was assessed by immunoblotting analysis. β-actin was used as a loading control.
2). Comprehensive Analysis of the Transcriptome-Wide m6A Methylation Modification Difference in Liver Fibrosis Mice by High-Throughput m6A Sequencing. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 34869362) [IF=4.6]

Application: WB    Species: Mice    Sample: Liver Fibrosis

FIGURE 7 Verification of m6A methylation level and methylase expression in LF. (A) The m6A methylation level in LF. (B) Semiquantitative analysis of m6A methylation. (C) IGV plots of the WTAP m6A level. (D) IGV plots of the ALKBH5 m6A level. (E) IGV plots of the YTHDF1 m6A level. (F) IGV plots of the WTAP expression level. (G) IGV plots of the ALKBH5 expression level. (H) IGV plots of the YTHDF1 expression level. (I) mRNA expression level of WTAP. (J) mRNA expression level of ALKBH5. (K) mRNA expression level of YTHDF1. (L) Protein expression levels of WTAP. (M) Protein expression levels of ALKBH5. (N) Protein expression levels of YTHDF1. (O) Semiquantitative analysis of WTAP protein. (P) Semiquantitative analysis of YTHDF1 protein. (Q) Semiquantitative analysis of ALKBH5 protein. ## p < 0.01 compared with the control group, # p < 0.05 compared with the control group.
3). Hypoxia triggers cardiomyocyte apoptosis via regulating the m6A methylation-mediated LncMIAT/miR-708-5p/p53 axis. Heliyon, 2024 (PubMed: 38961902) [IF=4.0]
4). HIF-1α-induced upregulation of m6A reader IGF2BP1 facilitates peripheral nerve injury recovery by enhancing SLC7A11 mRNA stabilization. In vitro cellular & developmental biology. Animal, 2023 (PubMed: 37783915) [IF=1.5]

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