製品: IGFBPL1 Antibody
カタログ: DF2512
タンパク質の説明: Rabbit polyclonal antibody to IGFBPL1
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Bovine, Sheep, Rabbit
分子量: 29 kDa(Observed); 29kD(Calculated).
ユニプロット: Q8WX77
RRID: AB_2839718

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
予測:
Bovine(89%), Sheep(%), Rabbit(%)
クローナリティ:
Polyclonal
特異性:
IGFBPL1 Antibody detects endogenous levels of total IGFBPL1.
RRID:
AB_2839718
引用形式: Affinity Biosciences Cat# DF2512, RRID:AB_2839718.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

bA113O24.1; IGFBP-related protein 10; IGFBP-RP4; IGFBPL1; IGFBPRP4; insulin-like growth factor binding protein related protein 4; insulin-like growth factor-binding protein-like 1; insulin-like growth factor-binding-related protein 4;

免疫原

免疫原:

A synthesized peptide derived from human IGFBPL1, corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
Q8WX77 IBPL1_HUMAN:

Expressed at the highest level in both brain and testis, with lower levels in the prostate, bladder and lung.

タンパク質配列:
MPRLSLLLPLLLLLLLPLLPPLSPSLGIRDVGGRRPKCGPCRPEGCPAPAPCPAPGISALDECGCCARCLGAEGASCGGRAGGRCGPGLVCASQAAGAAPEGTGLCVCAQRGTVCGSDGRSYPSVCALRLRARHTPRAHPGHLHKARDGPCEFAPVVVVPPRSVHNVTGAQVGLSCEVRAVPTPVITWRKVTKSPEGTQALEELPGDHVNIAVQVRGGPSDHEATAWILINPLRKEDEGVYQCHAANMVGEAESHSTVTVLDLSKYRSFHFPAPDDRM

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Bovine
89
Sheep
89
Pig
78
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

IGF-binding proteins prolong the half-life of IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs in cell culture. They alter the interaction of IGFs with their cell surface receptors (By similarity). May be a putative tumor suppressor protein.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at the highest level in both brain and testis, with lower levels in the prostate, bladder and lung.

参考文献

1). IGFBPL1 inhibits macrophage lipid accumulation by enhancing the activation of IGR1R/LXRα/ABCG1 pathway. Aging, 2023 (PubMed: 38157252) [IF=3.9]

Application: WB    Species: human    Sample: macrophages

Figure 1. IGFBPL1 inhibits PA-induced lipid accumulation in macrophages. (A) Different expression gene in control samples and unstable atherosclerosis plaque human. Normal: the abdominal aortas intima of three patients without atherosclerosis. Atherosclerosis: aortic plaques of patients diagnosed with grade V or VI atherosclerosis. (B) Relative IGFBPL1 protein level (n=3). (C) Relative mRNA level of IGFBPL1 in PA or ox-LDL treated THP-1 macrophages (n=3). (D) Relative protein level of IGFBPL1 (n=3). (E) Relative IGFBPL1 mRNA level (n=3). (F) Representative images of oil red O staining. PA: palmitic acid, THP-1 macrophages were incubated with 100 μM PA for 48 h. ox-LDL: oxidized low density lipoprotein, THP-1 macrophages were treated with 100 μg/mL ox-LDL for 72 h. PA + ov-IGFBPL1, THP-1 was transfected with over-expression plasmid of IGFBPL1 for 24 h and then incubated with 100 μM PA for 48 h. P

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For Research Use Only.
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