CITED2 Antibody - #DF2455

(2)   (4)  
製品: CITED2 Antibody
カタログ: DF2455
タンパク質の説明: Rabbit polyclonal antibody to CITED2
アプリケーション: WB IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Dog, Chicken
分子量: 28 kDa; 28kD(Calculated).
ユニプロット: Q99967
RRID: AB_2839661

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(82%)
クローナリティ:
Polyclonal
特異性:
CITED2 Antibody detects endogenous levels of total CITED2.
RRID:
AB_2839661
引用形式: Affinity Biosciences Cat# DF2455, RRID:AB_2839661.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ASD8; Cbp/p300 interacting transactivator 2; Cbp/p300 interacting transactivator with Glu/Asp rich carboxy terminal domain 2; Cbp/p300-interacting transactivator 2; CITE2_HUMAN; CITED 2; CITED2; melanocyte-specific gene 1-related gene 1; MRG 1; MRG-1; MRG1; MSG related protein 1; MSG-related protein 1; MSG1-related gene 1; P35srj; VSD2;

免疫原

免疫原:

A synthesized peptide derived from human CITED2, corresponding to a region within the internal amino acids.

Uniprot:

Q99967(CITE2_HUMAN) >>Visit HPA database.

遺伝子(ID):
CITED2(10370)
タンパク質の説明:
Interferes with the binding of transcription factors HIF-1a and STAT2 to p300/CBP.
タンパク質配列:
MADHMMAMNHGRFPDGTNGLHHHPAHRMGMGQFPSPHHHQQQQPQHAFNALMGEHIHYGAGNMNATSGIRHAMGPGTVNGGHPPSALAPAARFNNSQFMGPPVASQGGSLPASMQLQKLNNQYFNHHPYPHNHYMPDLHPAAGHQMNGTNQHFRDCNPKHSGGSSTPGGSGGSSTPGGSGSSSGGGAGSSNSGGGSGSGNMPASVAHVPAAMLPPNVIDTDFIDEEVLMSLVIEMGLDRIKELPELWLGQNEFDFMTDFVCKQQPSRVSC

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
82
Xenopus
78
Horse
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Transcriptional coactivator of the p300/CBP-mediated transcription complex. Acts as a bridge, linking TFAP2 transcription factors and the p300/CBP transcriptional coactivator complex in order to stimulate TFAP2-mediated transcriptional activation. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Stimulates the peroxisome proliferator-activated receptors PPARA transcriptional activity. Enhances estrogen-dependent transactivation mediated by estrogen receptors. Acts also as a transcriptional corepressor; interferes with the binding of the transcription factors HIF1A or STAT2 and the p300/CBP transcriptional coactivator complex. Participates in sex determination and early gonad development by stimulating transcription activation of SRY. Plays a role in controlling left-right patterning during embryogenesis; potentiates transcriptional activation of NODAL-mediated gene transcription in the left lateral plate mesoderm (LPM). Plays an essential role in differentiation of the adrenal cortex from the adrenogonadal primordium (AGP); stimulates WT1-mediated transcription activation thereby up-regulating the nuclear hormone receptor NR5A1 promoter activity. Associates with chromatin to the PITX2 P1 promoter region.

細胞の位置付け:

Nucleus.
Note: Colocalizes with EP300 in dot-like structures.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the CITED family.

参考文献

1). Poly-3-hydroxybutyrate-co-3-hydroxyvalerate(PHBV)-Polyethylene glycol 20k(PEG20k) as a promising delivery system for PT2399 in the treatment of disc degeneration. Journal of biological engineering, 2024 (PubMed: 38254196) [IF=5.7]

Application: WB    Species: human    Sample:

Fig. 6 Gene expression changes in nucleus pulposus cells treated with each experimental group for 48 hours were detected using RT-qPCR. (B) Western bolt showed protein expression levels in different experimental groups.Mean ± SD represents all the statistical data. The significance levels are denoted as follows: (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns indicates that the difference is not statistically significant)
2). CITED2 Mediates Metabolic Reprogramming in Renal Tubular Epithelial Cells via the AKT Signaling Pathway to Induce Sepsis-Associated Acute Kidney Injury. Journal of inflammation research, 2024 (PubMed: 39600684) [IF=4.5]

Application: IF/ICC    Species: Mouse    Sample:

Figure 1 Impairment of kidney structure and function in septic mice, characterized by macrophage infiltration and elevated CITED2 expression. (A-C). Serum expression levels of IL-1β, IL-6, and TNF-α in the Sham and Sepsis groups. (D and E). Serum levels of UREA and CREA in the Sham and Sepsis groups. (F and G). Gene expression levels of kidney injury markers (KIM-1 and NGAL) in kidney tissues of the Sham and Sepsis groups. H. HE staining of renal tissues in the Sham and Sepsis groups. (I). PAS staining of renal tissues in both Sham and Sepsis groups. (J). Protein and gene expression levels of CITED2 in renal tissues of the Sham and Sepsis groups. (K). Immunofluorescence staining for CITED2 and F4/80 in renal tissues of the Sham and Sepsis groups. *P < 0.05, **P < 0.01, ***P < 0.001. Significance indicators: ns (no significant difference).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.