TAF7L Antibody - #DF2403

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製品: TAF7L Antibody
カタログ: DF2403
タンパク質の説明: Rabbit polyclonal antibody to TAF7L
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Pig, Bovine, Sheep, Dog
分子量: 42 kDa; 53kD(Calculated).
ユニプロット: Q5H9L4
RRID: AB_2839610

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(88%), Bovine(88%), Sheep(88%), Dog(88%)
クローナリティ:
Polyclonal
特異性:
TAF7L Antibody detects endogenous levels of total TAF7L.
RRID:
AB_2839610
引用形式: Affinity Biosciences Cat# DF2403, RRID:AB_2839610.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Cancer/testis antigen 40; CT40; FLJ23157; RNA polymerase II TBP-associated factor subunit Q; TAF2Q; TAF7 like RNA polymerase II, TATA box binding protein (TBP) associated factor; Taf7l; TAF7L_HUMAN; TATA box binding protein-associated factor, RNA polymerase II, Q; TATA box-binding protein-associated factor 50 kDa; TBP associated factor, RNA polymerase II, Q; Transcription initiation factor TFIID 50 kDa subunit; Transcription initiation factor TFIID subunit 7-like;

免疫原

免疫原:
Uniprot:

Q5H9L4(TAF7L_HUMAN) >>Visit HPA database.

遺伝子(ID):
TAF7L(54457)
発現特異性:
Q5H9L4 TAF7L_HUMAN:

Testis-specific.

タンパク質の説明:
Probably functions as a spermatogensis-specific component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May play a role in spermatogenesis.
タンパク質配列:
MECPEGQLPISSENDSTPTVSTSEVTSQQEPQILVDRGSETTYESSADIAGDEGTQIPADEDTQTDADSSAQAAAQAPENFQEGKDMSESQDEVPDEVENQFILRLPLEHACTVRNLARSQSVKMKDKLKIDLLPDGRHAVVEVEDVPLAAKLVDLPCVIESLRTLDKKTFYKTADISQMLVCTADGDIHLSPEEPAASTDPNIVRKKERGREEKCVWKHGITPPLKNVRKKRFRKTQKKVPDVKEMEKSSFTEYIESPDVENEVKRLLRSDAEAVSTRWEVIAEDGTKEIESQGSIPGFLISSGMSSHKQGHTSSEYDMLREMFSDSRSNNDDDEDEDDEDEDEDEDEDEDEDKEEEEEDCSEEYLERQLQAEFIESGQYRANEGTSSIVMEIQKQIEKKEKKLHKIQNKAQRQKDLIMKVENLTLKNHFQSVLEQLELQEKQKNEKLISLQEQLQRFLKK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Bovine
88
Sheep
88
Dog
88
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5H9L4 基板として

Site PTM Type Enzyme
T184 Phosphorylation
T223 Phosphorylation
S258 Phosphorylation

研究背景

機能:

Probably functions as a spermatogenesis-specific component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May play a role in spermatogenesis (By similarity).

細胞の位置付け:

Nucleus. Cytoplasm.
Note: Cytoplasmic in spermatogonia and early spermatocytes (preleptotene, leptotene, and zygotene); translocates into the nuclei of pachytene spermatocytes and round spermatids.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Testis-specific.

サブユニット構造:

TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TAF7L may replace TAF7 in a spermatogenesis-specific form of TFIID. Interacts with TBP; the interaction occurs in a sub-population of cells (pachytene and haploid round spermatids) and is developmentally regulated through differential intracellular localization of the two proteins. Interacts with TAF1 (By similarity).

タンパク質ファミリー:

Belongs to the TAF7 family.

研究領域

· Genetic Information Processing > Transcription > Basal transcription factors.

参考文献

1). Genetic characterization of a missense mutation in the X-linked TAF7L gene identified in an oligozoospermic man. BIOLOGY OF REPRODUCTION (PubMed: 35554494) [IF=3.6]

Application: WB    Species: Mouse    Sample:

Figure 2 Generation of Taf7lD144G knockin mice. (A) Schematic of mouse Taf7l gene structure, targeting vector, and various alleles. Taf7l consists of 13 exons. Only the first nine exons are shown in rectangles. Exons 10 to 13 are not shown. The asterisk in Exon 4 denotes the point mutation. PGK-Neo encodes a neomycin selection marker. HyTK enables positive selection with hygromycin (not used) and negative selection with ganciclovir. pOG231 expresses Cre recombinase. (B) Genotyping of Taf7l alleles. (C) Sequencing of the wild type and Taf7lD144G alleles. (D) qRT-PCR analysis of the Taf7l transcript in wild type and Taf7lD144G/Y testes from 3-month-old mice. n.s.: nonsignificant, by Student’s t-test. (E) Western blot analysis of TAF7L in 3-month-old wild type and Taf7lD144G/Y testes. GAPDH serves as a loading control.

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