PHLDA3 Antibody - #DF2389

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製品: PHLDA3 Antibody
カタログ: DF2389
タンパク質の説明: Rabbit polyclonal antibody to PHLDA3
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Rabbit
分子量: 14 kDa; 14kD(Calculated).
ユニプロット: Q9Y5J5
RRID: AB_2839597

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MS Report
HPLC Report
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(90%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
PHLDA3 Antibody detects endogenous levels of total PHLDA3.
RRID:
AB_2839597
引用形式: Affinity Biosciences Cat# DF2389, RRID:AB_2839597.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

PHLA3_HUMAN; PHLDA 3; phlda3; Pleckstrin homology like domain family A member 2; Pleckstrin homology like domain family A member 3; Pleckstrin homology-like domain family A member 3; TDAG51/Ipl homolog 1; TDAG51/Ipl homologue 1; TIH 1; TIH1;

免疫原

免疫原:

A synthesized peptide derived from human PHLDA3, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9Y5J5(PHLA3_HUMAN) >>Visit HPA database.

遺伝子(ID):
PHLDA3(23612)
発現特異性:
Q9Y5J5 PHLA3_HUMAN:

Widely expressed with lowest expression in liver and spleen.

タンパク質の説明:
p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor.
タンパク質配列:
MTAAATATVLKEGVLEKRSGGLLQLWKRKRCVLTERGLQLFEAKGTGGRPKELSFARIKAVECVESTGRHIYFTLVTEGGGEIDFRCPLEDPGWNAQITLGLVKFKNQQAIQTVRARQSLGTGTLVS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
90
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor.

細胞の位置付け:

Cytoplasm. Membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed with lowest expression in liver and spleen.

タンパク質ファミリー:

The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.

Belongs to the PHLDA3 family.

参考文献

1). PHLDA3 exerts an antitumor function in prostate cancer by down-regulating Wnt/β-catenin pathway via inhibition of Akt. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2021 (PubMed: 34303965) [IF=2.5]

Application: WB    Species: Human    Sample: prostate cancer cells

Fig. 1. The expression and prognostic value of PHLDA3 in prostate cancer. The expression profile of PHLDA3 in prostate cancer was assessed via TCGA data through the online databases (A) GEPIA2 (*p < 0.05) and (B) UALCAN (***p < 0.001). (C) Levels of PHLDA3 mRNA in prostate cancer specimens were measured via RT-qPCR (***p < 0.001). (D). Protein levels of PHLDA3 in prostate cancer specimens were determined via western blotting (***p < 0.001). (E) Kaplan-Meier analysis of overall survival in prostate cancer patients with different levels of PHLDA3, obtained from the UALCAN database. (F) Expression levels of PHLDA3 in prostate cancer cell lines (DU-145, PC-3, LNCaP and 22Rv1) were examined via western blot (*p < 0.05 and **p < 0.01 vs. RWPE-1). (G) The promoter methylation level of PHLDA3 in prostate cancer was analyzed via TCGA data through the UALCAN database (****p < 0.0001). The demethylation compound 5-AZA-CdR was added to cells at a concentration of 10 m M, followed by incubation for 72 h. Cells treated with dimethylsulfoxide (DMSO) were used as a control. (H) The effects of 5-AZA-CdR treatment on the expression of PHLDA3 were assessed via western blot (**p < 0.01).

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