RAD1 Antibody - #DF2364
| 製品: | RAD1 Antibody |
| カタログ: | DF2364 |
| タンパク質の説明: | Rabbit polyclonal antibody to RAD1 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 32 kDa; 32kD(Calculated). |
| ユニプロット: | O60671 |
| RRID: | AB_2839572 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2364, RRID:AB_2839572.
折りたたみ／展開
Cell cycle checkpoint protein Hrad1; Cell cycle checkpoint protein Rad 1 A / B; Cell cycle checkpoint protein RAD1; Checkpoint control protein HRAD1; Checkpoint control protein RAD1; DNA repair exonuclease; DNA repair exonuclease rad1; DNA repair exonuclease rad1 homolog; DNA repair exonuclease REC1; DNA repair protein RAD1; EC 3.1.11.2; Exonuclease homolog RAD1; GTP-binding protein RAD; hRAD 1; hRAD1; MGC77779; RAD 1; RAD; RAD1; RAD1 homolog (S. pombe); RAD1 homolog; Rad1 like DNA damage checkpoint; Rad1 like DNA damage checkpoint protein; RAD1, S. pombe, homolog of; Rad1-like DNA damage checkpoint protein; RAD1_HUMAN; Ras associated with diabetes; REC 1; REC1; RRAD;
免疫原
Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
- O60671 RAD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESVTFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLMLFLEEGGVVTVCKINTQEPEETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQITMSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKPSTKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPESES
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O60671 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T5 | Phosphorylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| K233 | Ubiquitination | Uniprot | |
| S280 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S282 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
研究背景
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
Nucleus.
Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD1 interacts with POLB, FEN1, HUS1, HUS1B, RAD9A and RAD9B. Interacts with DNAJC7.
Belongs to the rad1 family.
研究領域
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
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