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| 製品: | CENPM Antibody |
| カタログ: | DF2314 |
| タンパク質の説明: | Rabbit polyclonal antibody to CENPM |
| アプリケーション: | WB IHC |
| Cited expt.: | WB |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Sheep, Dog |
| 分子量: | 20kDa(Observed); 20kD(Calculated). |
| ユニプロット: | Q9NSP4 |
| RRID: | AB_2839538 |
Control Products
製品説明
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2314, RRID:AB_2839538.
折りたたみ／展開
bK250D10.2; C22orf18; CENP M; Centromere protein M; ICEN 39; ICEN39; Interphase centromere complex protein 39; MGC861; OTTHUMP00000028741; PANE 1; PANE1; Proliferation associated nuclear element 1; Proliferation associated nuclear element protein 1;
免疫原
A synthesized peptide derived from human CENPM, corresponding to a region within the internal amino acids.
Isoform 3 is highly expressed in spleen, and intermediately in heart, prostate and ovary. Isoform 3 is highly expressed in resting CD19 B-cells and B-lineage chronic lymphocytic leukemia (B-CLL) cells and weakly expressed in activated B-cells. Isoform 1 is selectively expressed in activated CD19 cells and weakly in resting CD19 B-cells.
- Q9NSP4 CENPM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNRPRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKLAHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
研究背景
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres.
Nucleus. Cytoplasm. Chromosome>Centromere>Kinetochore.
Note: Nuclear in non-confluent cells and cytoplasmic in confluent or dividing cells (By similarity). Localizes in the kinetochore domain of centromeres.
Isoform 3 is highly expressed in spleen, and intermediately in heart, prostate and ovary. Isoform 3 is highly expressed in resting CD19 B-cells and B-lineage chronic lymphocytic leukemia (B-CLL) cells and weakly expressed in activated B-cells. Isoform 1 is selectively expressed in activated CD19 cells and weakly in resting CD19 B-cells.
参考文献
Application: WB Species: human Sample: PANC-1 and CFPAC-1 cells
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