Control Products
製品説明
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2231, RRID:AB_2839462.
折りたたみ／展開
MGC132428; Ret finger protein-like 1; RFPL1; RFPL1_HUMAN; RING finger protein 78; RNF78;
免疫原
A synthesized peptide derived from human RFPL1, corresponding to a region within the internal amino acids.
Seems to be expressed in prostate and less abundantly in adult brain, fetal liver, and fetal kidney.
- O75677 RFPL1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKRLSLVTTNRLSPHGNFLPLCTFPLAVDMAALFQEASSCPVCSDYLEKPMSLECGCAVCFKCINSLQKEPHGEDLLCCCCSMVSQKNKIRPSWQLERLASHIKELEPKLKKILQMNPRMRKFQVDMTLDADTANNFLLISDDLRSVRSGCITQNRQDLAERFDVSICILGSPRFTCGRHYWEVDVGTSTEWDLGVCRESVHRKGRIHLTTERGFWTVSLRDGSRLSASTVPLTFLFVDRKLQRVGIFLDMGMQNVSFFDAEGGSHVYTFRSVSAEEPLHLFFAPPSPPNGDKSVLSICPVINPGTTDAPVHPGEAK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
研究背景
Negatively regulates the G2-M phase transition, possibly by promoting cyclin B1/CCNB1 and CDK1 proteasomal degradation and thereby preventing their accumulation during interphase.
Phosphorylated by PKC and CDK1. The antiproliferative effect seems to be positively regulated by PKC phosphorylation and negatively by CDK1 phosphorylation.
Cytoplasm. Nucleus.
Note: A higher concentration is observed in the cytoplasm compared to the nucleus.
Seems to be expressed in prostate and less abundantly in adult brain, fetal liver, and fetal kidney.
The B30.2/SPRY domain is required for the negative regulation of cell proliferation.
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