EGF Antibody - #DF2225

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製品: EGF Antibody
カタログ: DF2225
タンパク質の説明: Rabbit polyclonal antibody to EGF
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Horse, Rabbit, Dog
分子量: 78 kDa; 134kD(Calculated).
ユニプロット: P01133
RRID: AB_2839456

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(88%), Horse(88%), Rabbit(88%), Dog(88%)
クローナリティ:
Polyclonal
特異性:
EGF Antibody detects endogenous levels of total EGF.
RRID:
AB_2839456
引用形式: Affinity Biosciences Cat# DF2225, RRID:AB_2839456.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Beta urogastrone; beta-urogastrone; EGF; EGF_HUMAN; Epidermal growth factor; HOMG4; OTTHUMP00000219721; OTTHUMP00000219722; Pro epidermal growth factor; URG; Urogastrone;

免疫原

免疫原:

A synthesized peptide derived from human EGF, corresponding to a region within the internal amino acids.

Uniprot:

P01133(EGF_HUMAN) >>Visit HPA database.

遺伝子(ID):
EGF(1950)
発現特異性:
P01133 EGF_HUMAN:

Expressed in kidney, salivary gland, cerebrum and prostate.

タンパク質の説明:
Could be involved in cardiac development
タンパク質配列:
MLLTLIILLPVVSKFSFVSLSAPQHWSCPEGTLAGNGNSTCVGPAPFLIFSHGNSIFRIDTEGTNYEQLVVDAGVSVIMDFHYNEKRIYWVDLERQLLQRVFLNGSRQERVCNIEKNVSGMAINWINEEVIWSNQQEGIITVTDMKGNNSHILLSALKYPANVAVDPVERFIFWSSEVAGSLYRADLDGVGVKALLETSEKITAVSLDVLDKRLFWIQYNREGSNSLICSCDYDGGSVHISKHPTQHNLFAMSLFGDRIFYSTWKMKTIWIANKHTGKDMVRINLHSSFVPLGELKVVHPLAQPKAEDDTWEPEQKLCKLRKGNCSSTVCGQDLQSHLCMCAEGYALSRDRKYCEDVNECAFWNHGCTLGCKNTPGSYYCTCPVGFVLLPDGKRCHQLVSCPRNVSECSHDCVLTSEGPLCFCPEGSVLERDGKTCSGCSSPDNGGCSQLCVPLSPVSWECDCFPGYDLQLDEKSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVYALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAVDWIGRRFYWTDRGKSLIGRSDLNGKRSKIITKENISQPRGIAVHPMAKRLFWTDTGINPRIESSSLQGLGRLVIASSDLIWPSGITIDFLTDKLYWCDAKQSVIEMANLDGSKRRRLTQNDVGHPFAVAVFEDYVWFSDWAMPSVMRVNKRTGKDRVRLQGSMLKPSSLVVVHPLAKPGADPCLYQNGGCEHICKKRLGTAWCSCREGFMKASDGKTCLALDGHQLLAGGEVDLKNQVTPLDILSKTRVSEDNITESQHMLVAEIMVSDQDDCAPVGCSMYARCISEGEDATCQCLKGFAGDGKLCSDIDECEMGVPVCPPASSKCINTEGGYVCRCSEGYQGDGIHCLDIDECQLGEHSCGENASCTNTEGGYTCMCAGRLSEPGLICPDSTPPPHLREDDHHYSVRNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRHAGHGQQQKVIVVAVCVVVLVMLLLLSLWGAHYYRTQKLLSKNPKNPYEESSRDVRSRRPADTEDGMSSCPQPWFVVIKEHQDLKNGGQPVAGEDGQAADGSMQPTSWRQEPQLCGMGTEQGCWIPVSSDKGSCPQVMERSFHMPSYGTQTLEGGVEKPHSLLSANPLWQQRALDPPHQMELTQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
88
Dog
88
Rabbit
88
Chicken
75
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro.

PTMs:

O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).

細胞の位置付け:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in kidney, salivary gland, cerebrum and prostate.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

参考文献

1). An in vitro and in vivo study of the brain-targeting effects of an epidermal growth factor-functionalized cholera toxin-like chimeric protein. JOURNAL OF CONTROLLED RELEASE, 2020 (PubMed: 32205153) [IF=10.5]

Application: WB    Species: Mice    Sample: Brain

Fig. 1. Expression, purification and identification of proteins. A) Plasmid map of pET22b-EGF-CTA2-TAT. B) Plasmid map of pET28b-CTB. C) Expression of the EGFCTA2-TAT (15.7 kDa) protein and D) CTB (12.8 kDa), as indicated by SDS-PAGE. M: Marker; 1: uninduced BL21(DE3); 2: induced BL21(DE3); 3: supernatant after sonication; and 4: pellet after sonication. E) Results from SDS-PAGE and western blot analysis of the purified EGF-CTA2-TAT and CTB; 1: purified CTB; 2: purified EGF-CTA2-TAT; 3, 4: results from the western blot analysis of the purified CTB and EGF-CTA2-TAT using anti-EGF antibody; and 5, 6: results from the western blot analysis of the purified CTB and EGF-CTA2-TAT proteins using anti-CTB antibody. F) Identification of the (CTB)5 (64.0 kDa) and EGF-CTA2-TAT/(CTB)5 (79.7 kDa) proteins by native-PAGE. M: Marker; 1: (CTB)5; and 2: EGF-CTA2-TAT/(CTB)5.
2). Pien-tze-huang promotes wound healing in streptozotocin-induced diabetes models associated with improving oxidative stress via the Nrf2/ARE pathway. Frontiers in pharmacology, 2023 (PubMed: 36713837) [IF=5.6]

Application: WB    Species: Rat    Sample:

FIGURE 3. PZH increased the expression of growth factors in diabetic wounds. (A–C) Effect of topical treatment with PZH on the eighth day of wound healing at the mRNA levels of c-jun (A), PDGF (B), and VEGF-A (C) in diabetic rat wounds. *p < 0.05, **p < 0.01 (n = 3). (D–H) The protein level of c-jun, VEGF-A, PDGF, and EGF was detected in type 1 diabetic rat wounds on the eighth day of topical treatment with PZH, (D) is representative blotting, band intensity was quantified using ImageJ, right panels are quantification of c-jun (E), PDGF (F), VEGF-A (G) and EGF (H) level, *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3). (I–L) The protein level of PDGF (J), VEGF-A (K), and EGF (L) were detected in type 2 diabetic mouse wounds on the 10th day of PZH gavage treatment by Western blotting, and band intensity was quantified using ImageJ, (I) is representative blotting. *p < 0.05, **p < 0.01 (n = 4). Data are expressed as the mean ± SD. DM: diabetic control group; PZH: PZH-treated group.
3). Effects of endometrial stem cell transplantation combined with estrogen in the repair of endometrial injury. Oncology Letters, 2018 (PubMed: 29963188) [IF=2.5]

Application: WB    Species: rat    Sample: endometrium

Figure 1. |The mRNA and protein expression levels of EGF and PDGF‑BB in endometrial tissue. (A) mRNA levels of EGF and PDGF‑BB in endometrial tissue. (B) Protein expression levels of EGF and PDGF‑BB in endometrial tissue. *P<0.05 vs. control. EGF, epidermal growth factor; PDGF‑BB, platelet‑derived growth factor‑BB; IUA, intrauterine adhesions.

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