Tubulin alpha Antibody - #T0033

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製品: Tubulin alpha Antibody
カタログ: T0033
タンパク質の説明: Mouse monoclonal antibody to Tubulin alpha
アプリケーション: WB ELISA
Cited expt.: WB
反応性: Human, Mouse, Zebrafish, Rabbit
分子量: 55KD; 50kD(Calculated).
ユニプロット: P68366
RRID: AB_2839424

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IHC Protocol
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製品説明

ソース:
Mouse
アプリケーション:
WB 1:3000-1:10000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Zebrafish,Rabbit
クローナリティ:
Monoclonal [F794]
特異性:
The α-tubulin antibody can detects ebrafish endogenousα-tubulin protein.
RRID:
AB_2839424
引用形式: Affinity Biosciences Cat# T0033, RRID:AB_2839424.
コンジュゲート:
Unconjugated.
精製:
affinity purification.
保存:
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

TUBA4A, Alpha-tubulin 1, TUBA1, Testis-specific alpha-tubulin, Tubulin, alpha 4a, H2-ALPHA, Tubulin alpha-1 chain, Tubulin alpha-4 chain, Tubulin alpha-4A chain, Tubulin H2-alpha, Tubulin, alpha 1

免疫原

免疫原:

Peptide from Zebrafish alpha tubulin.

Uniprot:

P68366(TBA4A_HUMAN) >>Visit HPA database.

遺伝子(ID):
TUBA4A(7277)
タンパク質の説明:
Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 KD. Microtubules are assembled from dimers of α- and β-tubulin.
タンパク質配列:
MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE

研究背景

機能:

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

PTMs:

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.

Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).

Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.

Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.

細胞の位置付け:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the tubulin family.

研究領域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

参考文献

1). Downregulation of OPN3 Gene Induces Ferroptosis, Apoptosis, and Pyroptosis of Human Dermal Fibroblasts In Vitro. The Journal of investigative dermatology, 2024 (PubMed: 38548258) [IF=5.7]
2). Protection of Ficus pandurata Hance against acute alcohol-induced liver damage in mice via suppressing oxidative stress, inflammation, and apoptosis. Journal of Ethnopharmacology, 2021 (PubMed: 33915134) [IF=4.8]

Application: WB    Species: mice    Sample: liver tissues

Fig. 5. Effects of FPHAP on the oxidative stress and Nrf2/HO-1 signaling pathway of liver tissues in acute ALD mice. (A) Levels of ROS, MDA, SOD and GSH, (B) Protein expressions of Nrf2, HO-1, NQO-1 in liver tissues of mice treated with or without Silybin, FPHAP (12 g/kg), FPHAP (24 g/kg), and FPHAP (48 g/kg). Data (n = 8) are expressed as mean ± SEM. ##p < 0.01, ###p < 0.001, comparing with normal; *p < 0.05，**p < 0.01, ***p < 0.001, comparing with model.

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