製品: ATP1B1 Antibody
カタログ: DF7473
タンパク質の説明: Rabbit polyclonal antibody to ATP1B1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 35kDa; 35kD(Calculated).
ユニプロット: P05026
RRID: AB_2839410

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(92%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(83%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
ATP1B1 Antibody detects endogenous levels of total ATP1B1.
RRID:
AB_2839410
引用形式: Affinity Biosciences Cat# DF7473, RRID:AB_2839410.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Adenosinetriphosphatase; AT1B1_HUMAN; ATP 1B; ATP1B; ATP1B1; ATPase Na+/K+ transporting beta 1 polypeptide; Atpb 1; ATPBS; Beta 1 subunit of Na(+) K(+) ATPase; Na K ATPase beta 1 polypeptide; Na+/K+ ATPase beta 1 subunit; Sodium/potassium dependent ATPase beta 1 subunit; Sodium/potassium transporting ATPase beta 1 chain; Sodium/potassium transporting ATPase subunit beta 1; Sodium/potassium-dependent ATPase subunit beta-1; Sodium/potassium-transporting ATPase subunit beta-1;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P05026 AT1B1_HUMAN:

Found in most tissues.

タンパク質の説明:
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known.
タンパク質配列:
MARGKAKEEGSWKKFIWNSEKKEFLGRTGGSWFKILLFYVIFYGCLAGIFIGTIQVMLLTISEFKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIVRFLEKYKDSAQRDDMIFEDCGDVPSEPKERGDFNHERGERKVCRFKLEWLGNCSGLNDETYGYKEGKPCIIIKLNRVLGFKPKPPKNESLETYPVMKYNPNVLPVQCTGKRDEDKDKVGNVEYFGLGNSPGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTMDTEIRIECKAYGENIGYSEKDRFQGRFDVKIEVKS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Pig
92
Bovine
91
Sheep
91
Rabbit
83
Chicken
75
Xenopus
58
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05026 基板として

Site PTM Type Enzyme
K13 Ubiquitination
K14 Ubiquitination
K21 Ubiquitination
K85 Ubiquitination
K96 Ubiquitination
Y101 Phosphorylation
K113 Ubiquitination
R118 Methylation
K134 Ubiquitination
K147 Ubiquitination
K152 Ubiquitination
N158 N-Glycosylation
K170 Ubiquitination
K173 Ubiquitination
K179 Ubiquitination
K187 Ubiquitination
N193 N-Glycosylation
K216 Ubiquitination
K221 Ubiquitination
K223 Ubiquitination
Y229 Phosphorylation
S235 Phosphorylation
K248 Ubiquitination
Y254 Phosphorylation
N265 N-Glycosylation
K277 Ubiquitination
Y285 Phosphorylation
K288 Ubiquitination

研究背景

機能:

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.

Involved in cell adhesion and establishing epithelial cell polarity.

PTMs:

Glutathionylated.

細胞の位置付け:

Cell membrane>Single-pass type II membrane protein. Cell membrane>Sarcolemma.
Note: Colocalizes with OBSCN at the intercalated disk and sarcolemma in cardiomyocytes. Localizes in long striations at the level of Z and M lines.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Found in most tissues.

サブユニット構造:

The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit. Interacts with regulatory subunit FXYD1 (By similarity). Interacts with regulatory subunit FXYD3 (By similarity). Interacts with NKAIN1, NKAIN2 and NKAIN4 (By similarity). Interacts with MLC1. Part of a complex containing MLC1, TRPV4, AQP4 and HEPACAM. Interacts with KIRREL3. Interacts with OBSCN (via protein kinase domain 1) (By similarity).

タンパク質ファミリー:

The C-terminal lobe folds into an immunoglobulin-like domain and mediates cell adhesion properties.

Belongs to the X(+)/potassium ATPases subunit beta family.

研究領域

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.

· Organismal Systems > Digestive system > Protein digestion and absorption.

· Organismal Systems > Digestive system > Mineral absorption.

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