CALCA Antibody - #DF7386

(3)   (5)  
製品: CALCA Antibody
カタログ: DF7386
タンパク質の説明: Rabbit polyclonal antibody to CALCA
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Sheep, Dog, Chicken
分子量: 13kDa; 14kD(Calculated).
ユニプロット: P06881
RRID: AB_2839324

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(83%), Zebrafish(89%), Sheep(83%), Dog(92%), Chicken(83%)
クローナリティ:
Polyclonal
特異性:
CALCA Antibody detects endogenous levels of total CALCA.
RRID:
AB_2839324
引用形式: Affinity Biosciences Cat# DF7386, RRID:AB_2839324.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Calcitonin gene-related peptide 1;Alpha-type CGRP;Calcitonin gene-related peptide I;CGRP-I;CALCA;CALC1;

免疫原

免疫原:

A synthesized peptide derived from human CALCA, corresponding to a region within the internal amino acids.

Uniprot:

P06881(CALCA_HUMAN) >>Visit HPA database.

遺伝子(ID):
CALCA(796)
タンパク質の説明:
This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.
タンパク質配列:
MGFQKFSPFLALSILVLLQAGSLHAAPFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSRIIAQKRACDTATCVTHRLAGLLSRSGGVVKNNFVPTNVGSKAFGRRRRDLQA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
92
Zebrafish
89
Pig
83
Sheep
83
Chicken
83
Horse
0
Bovine
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the calcitonin family.

参考文献

1). Multi-omics and network pharmacology approaches reveal the mechanism of action of KeKe tablet against post-infectious cough. Chinese medicine, 2025 (PubMed: 41044788) [IF=5.3]
2). Danggui niantong decoction attenuates synovial fibrosis through regulating PI3k/AKT signaling pathway. Journal of ethnopharmacology, 2025 (PubMed: 39832630) [IF=4.8]
3). Activation of oxytocin receptor in the trigeminal ganglion attenuates orofacial ectopic pain attributed to inferior alveolar nerve injury. Journal of Neurophysiology, 2021 (PubMed: 33326336) [IF=2.1]

Application: IF/ICC    Species: Rat    Sample:

Figure 2.Oxytocin receptor (OXTR) wasexpressed in neurons but not glial cells inthe trigeminal ganglion (TG) and spinal tri-geminal nucleus caudalis (SpVc) of inferioralveolar nerve (IAN) transection (IANX)rats.A: in TG slices, arrowheads showcolocalization of NeuN (neuron marker)and OXTR (red, NeuN; green, OXTR; blue,DAPI).B: in TG slices, activated satelliteglial cells (arrows) surrounded OXTR-posi-tive neurons (arrowheads), and there wasno coexpression [red, glialfibrillary acidicprotein (GFAP); green, OXTR; blue, DAPI].C: in TG slices, coexpression of OXTR andcalcitonin gene-related peptide (CGRP) isindicated with arrowheads (red, CGRP;green, OXTR; blue, DAPI).D:inSpVcsli-ces, double immunohistochemistry indi-cates colocalization of NeuN and OXTR(arrowheads: red, NeuN; green, OXTR;blue, DAPI).E:inSpVcslices,immunofluo-rescence (IF) staining shows no OXTR(arrowheads) colocalized with GFAP(arrows: red, GFAP; green, OXTR; blue,DAPI) (n= 3 rats in each group; scale bars,50μm).INFLUENCE OF OXYTOCIN RECEPTOR ON OROFACIAL ECTOPIC PAINJ Neurophysioldoi:10.1152/jn.00646.2020www.jn.org227

Application: WB    Species: Rat    Sample:

Figure 3.Inferior alveolar nerve (IAN) transection (IANX) resulted in upregulated expression of oxytocin receptor (OXTR), calcitonin gene-related peptide(CGRP), IL-1b, and TNFain the trigeminal ganglion (TG) ondays 1, 4,and7after surgery.AandD: representative Western blot (WB) bands of OXTR,CGRP, IL-1b, and TNFaexpression in the trigeminal ganglion (TG) ondays 1, 4,and7after operation.B, C, E,andF: bar graphs show increased OXTR,CGRP, IL-1band TNFaexpression of IANX rats vs. 2 other groups; sham operation did not result in significant change vs. naive rats (P

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.