S100A11 Antibody - #DF7368

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製品: S100A11 Antibody
カタログ: DF7368
タンパク質の説明: Rabbit polyclonal antibody to S100A11
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 11kDa; 12kD(Calculated).
ユニプロット: P31949
RRID: AB_2839306

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(83%)
クローナリティ:
Polyclonal
特異性:
S100A11 Antibody detects endogenous levels of total S100A11.
RRID:
AB_2839306
引用形式: Affinity Biosciences Cat# DF7368, RRID:AB_2839306.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Calgizzarin; Epididymis secretory protein Li 43; HEL S 43; Metastatic lymph node gene 70 protein; MLN 70; MLN70; Protein S100 A11; Protein S100-A11, N-terminally processed; Protein S100-C; Protein S100A11; Protein S100C; S100 A11; S100 calcium binding protein A11; S100 calcium-binding protein A11 (calgizzarin); S100 calcium-binding protein A11; S100a11; S100C; S10AB_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human S100A11, corresponding to a region within the internal amino acids.

Uniprot:

P31949(S10AB_HUMAN) >>Visit HPA database.

遺伝子(ID):
S100A11(6282)
タンパク質の説明:
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis.
タンパク質配列:
MAKISSPTETERCIESLIAVFQKYAGKDGYNYTLSKTEFLSFMNTELAAFTKNQKDPGVLDRMMKKLDTNSDGQLDFSEFLNLIGGLAMACHDSFLKAVPSQKRT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
92
Bovine
92
Sheep
92
Chicken
83
Xenopus
64
Zebrafish
55
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Facilitates the differentiation and the cornification of keratinocytes.

PTMs:

Phosphorylation at Thr-10 by PRKCA significantly suppresses homodimerization and promotes association with NCL/nucleolin which induces nuclear translocation.

細胞の位置付け:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the S-100 family.

参考文献

1). CAV2 promotes the invasion and metastasis of head and neck squamous cell carcinomas by regulating S100 proteins. Cell death discovery, 2022 (PubMed: 36114176) [IF=7.0]

Application: WB    Species: human    Sample: SCC15 and SCC25 cells

Fig. 3: The S100 protein family affects HNSCC metastasis and is regulated by CAV2. A The expression of S100 family proteins in CAV2-control and CAV2-knockdown (shCAV2-1, shCAV2-2) SCC15 and SCC25 HNSCC cell lines was evaluated by immunoblotting. B The expression of S100 family proteins in CAV2-control and CAV2-knockdown (shCAV2-1, shCAV2-2) SCC15 cell lines was evaluated by RT–qPCR. C Alterations in the invasive ability of SCC15 and SCC25 cells following siRNA interference of S100 protein family members. The invasive ability of SCC15 and SCC25 cells was detected using Transwell assays. *, **, *** and **** indicate p 

Application: IHC    Species: human    Sample: SCC15 and SCC25 cells

Fig. 3: The S100 protein family affects HNSCC metastasis and is regulated by CAV2. A The expression of S100 family proteins in CAV2-control and CAV2-knockdown (shCAV2-1, shCAV2-2) SCC15 and SCC25 HNSCC cell lines was evaluated by immunoblotting. B The expression of S100 family proteins in CAV2-control and CAV2-knockdown (shCAV2-1, shCAV2-2) SCC15 cell lines was evaluated by RT–qPCR. C Alterations in the invasive ability of SCC15 and SCC25 cells following siRNA interference of S100 protein family members. The invasive ability of SCC15 and SCC25 cells was detected using Transwell assays. *, **, *** and **** indicate p 

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