NQO2 Antibody - #DF7342

Figure 1 Selective expression of MT3Rs in MEC stellate neurons and elevated levels of melatonin within the MEC during the inactive phase. a) Representative images showing the expression of MT1Rs and MT2Rs in the MEC. Scale bar: 100 µm. b) Representative images showing the expression of MT3Rs in the reelin+ stellate neurons and calbindin+ pyramidal neurons of the MEC. Scale bar: 100 µm. c) Percentage of reelin+ stellate neurons and calbindin+ pyramidal neurons that were positive for MT3Rs (mean ± SEM). Mann-Whitney Rank Sum Test, **P < 0.01, n = 6 sections from 3 rats. d) Concentration of melatonin in the pineal gland tissue at 4 time points (10 A.M. and 11 A.M. represent inactive phase, 10 P.M. and 11 P.M. represent active phase). e) Bar graphs with overlaid dot plots showing the average concentration of melatonin in the pineal gland tissue during the day (10 A.M. and 11 A.M.) and night (10 P.M. and 11 P.M.) (mean ± SEM). Welch's t-test, *P < 0.05, n = 9 rats for each group. f) Concentration of CSF melatonin taken through the foramen magnum at 4 time points (10 A.M., 11 A.M., 10 P.M., and 11 P.M.). g) Bar graphs with overlaid dot plots showing the average concentration of the CSF melatonin during the day (10 A.M. and 11 A.M.) and night (10 P.M. and 11 P.M.) (mean ± SEM). Mann-Whitney Rank Sum Test, **P < 0.01, n = 10 rats for each group. h) Melatonin measurements in tissue homogenates of the MEC at 4 time points (10 A.M., 11 A.M., 10 P.M., and 11 P.M.). i) Bar graphs with overlaid dot plots showing the average concentration of melatonin in the MEC tissue homogenates during the day (10 A.M. and 11 A.M.) and night (10 P.M and 11 P.M.) (mean ± SEM). Welch's t-test, *P < 0.05, n = 15 sample points, inactive phase, n = 16 sample points, active phase, each sample point consists of bilateral MEC tissue samples from 3 rats. j) Images showing AANAT expression levels in the MEC (left), Scale bar: 50 µm. Bar graphs with overlaid dot plots showing the AANAT expression levels at active and inactive phases (mean ± SEM). Mann-Whitney Rank Sum Test, **P < 0.01, n = 12 sections from 4 rats, 12 A.M., n = 12 sections from 4 rats, 12 P.M. (right). k) Immunoblot analysis of AANAT expression levels at active and inactive phase (mean ± SEM). Student's t-test, *P < 0.05, n = 4 rats, 12 A.M., n = 4 rats, 12 P.M. MT1Rs, Melatonin type 1 receptors; MT2Rs, Melatonin type 2 receptors; MT3Rs, Melatonin type 3 receptors; MEC, medial entorhinal cortex; CSF, cerebrospinal fluid; AANAT, aralkylamine N-acetyltransferase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Fig. 3. |Idebenone inhibited NLRP3 signaling in BV2 cells under OGD/R conditions. (a) Immunoblot analysis of NQO1, NQO2, NOX2 and NLRP3 signaling in BV2 cells with or without treatment of idebenone after OGD/R.