CHI3L1 Antibody - #DF7223

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製品: CHI3L1 Antibody
カタログ: DF7223
タンパク質の説明: Rabbit polyclonal antibody to CHI3L1
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Rabbit, Dog
分子量: 43kD; 43kD(Calculated).
ユニプロット: P36222
RRID: AB_2839164

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MS Report
HPLC Report
MSDS
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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:100
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(83%), Bovine(92%), Rabbit(92%), Dog(92%)
クローナリティ:
Polyclonal
特異性:
CHI3L1 Antibody detects endogenous levels of total CHI3L1.
RRID:
AB_2839164
引用形式: Affinity Biosciences Cat# DF7223, RRID:AB_2839164.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

39 kDa synovial protein; ASRT7; Cartilage glycoprotein 39; CGP-39; CGP39; CH3L1_HUMAN; CHI3L1; chitinase 3 like 1 (cartilage glycoprotein 39); chitinase 3 like 1; Chitinase 3 like protein 1 precursor; chitinase; Chitinase-3-like protein 1; Chondrocyte protein YKL40; GP 39; GP-39; GP39; HC gp39; HCGP 3P; hCGP-39; HCgp39; YKL 40; YKL-40; YKL40; YYL 40;

免疫原

免疫原:

A synthesized peptide derived from human CHI3L1, corresponding to a region within the internal amino acids.

Uniprot:

P36222(CH3L1_HUMAN) >>Visit HPA database.

遺伝子(ID):
CHI3L1(1116)
発現特異性:
P36222 CH3L1_HUMAN:

Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.

タンパク質の説明:
Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.
タンパク質配列:
MGVKASQTGFVVLVLLQCCSAYKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
92
Dog
92
Rabbit
92
Pig
83
Sheep
75
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.

PTMs:

Glycosylated.

細胞の位置付け:

Secreted>Extracellular space. Cytoplasm. Cytoplasm>Perinuclear region. Endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.

タンパク質ファミリー:

Belongs to the glycosyl hydrolase 18 family.

参考文献

1). CHI3L1: a novel regulator of vascular remodeling in pulmonary arterial hypertension. Molecular and cellular biochemistry, 2026 (PubMed: 41910909) [IF=3.5]
2). Host-defence caerin 1.1 and 1.9 peptides suppress glioblastoma U87 and U118 cell proliferation through the modulation of mitochondrial respiration and induce the downregulation of CHI3L1. PloS one, 2024 (PubMed: 38848430) [IF=2.9]

Application: WB    Species: human    Sample: U87 cells

Fig 7. F1/F3 treatment inhibited the expression of CHI3L1 and shifted the energy metabolism of U87 cells. At 5 μg/mL, F1/F3 downregulated the content of CHI3L1 significantly (A) and induced a concentration-dependent response (B) in U87 cells. At 5 μg/mL, F1/F3 significantly downregulated the content of CHI3L1 (C) in U118 cells, and the concentration dependence displayed in (D). (E) The TCGA glioblastoma multiforme database revealed a notable upregulation of CHI3L1 in tumours compared to normal tissues. (F) Low CHI3L1 expression was positively correlated with the survival time of glioblastoma multiforme patients, showing statistical significance. (G) Evaluation of glycolysis/OXPHOS levels in the U87 (top) and U87F (bottom) groups demonstrated that F1/F3 treatment led to a shift from glycolysis to OXPHOS in U87 cells, as indicated by changes in ATP levels and lactate production. The inhibition of glycolysis or OXPHOS pathways was facilitated using 2-DG or oligomycin, respectively. Data are representative of at least 3 independent experiments and are presented as the mean ± SD.
3). The traditional Chinese patented medicine Qingke Pingchuan granules alleviate acute lung injury by regenerating club cells. Pulmonary circulation, 2022 (PubMed: 36186720) [IF=2.2]

Application: WB    Species: Mouse    Sample:

Figure 4 Validation of Scgb1a1, Sftpc, and Chi3l1 gene expression. (a) mRNA levels of Scgb1a1, Sftpc, and Chi3l1. (b) CC10, SFTPC, and CHI3L1 levels assessed with western blot. (c) IHC staining of CC10 and SFTPC. The red arrows indicate CC10 and SFTPC expression expression. (d) Quantitation of western blot. (e) Relative expression comparison of CC10 and SFTPC in IHC staining. ****p 

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