AIF1/IBA1 Antibody - #DF7217

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製品: AIF1/IBA1 Antibody
カタログ: DF7217
タンパク質の説明: Rabbit polyclonal antibody to AIF1/IBA1
アプリケーション: WB IHC IF/ICC
Cited expt.: IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit
分子量: 17kDa; 17kD(Calculated).
ユニプロット: P55008
RRID: AB_2839160

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MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:100, IF/ICC 1:100-500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
AIF1/IBA1 Antibody detects endogenous levels of total AIF1/IBA1.
RRID:
AB_2839160
引用形式: Affinity Biosciences Cat# DF7217, RRID:AB_2839160.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AIF 1; AIF-1; Aif1; AIF1 protein; AIF1_HUMAN; Allograft inflammatory factor 1; Allograft inflammatory factor 1 splice variant G; allograft inflammatory factor-1 splice variant Hara-1; balloon angioplasty responsive transcription; BART 1; G1; G1 putative splice variant of allograft inflamatory factor 1; IBA 1; IBA1; interferon gamma responsive transcript; Interferon responsive transcript 1; interferon responsive transcript factor 1; Ionized calcium binding adapter molecule 1; Ionized calcium-binding adapter molecule 1; ionized calcium-binding adapter molecule; IRT 1; IRT1; Microglia response factor; MRF1; Protein g1;

免疫原

免疫原:

A synthesized peptide derived from human AIF1/IBA1, corresponding to a region within C-terminal amino acids.

Uniprot:

P55008(AIF1_HUMAN) >>Visit HPA database.

遺伝子(ID):
AIF1(199)
発現特異性:
P55008 AIF1_HUMAN:

Detected in T-lymphocytes and peripheral blood mononuclear cells.

タンパク質の説明:
This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene.
タンパク質配列:
MSQTRDLQGGKAFGLLKAQQEERLDEINKQFLDDPKYSSDEDLPSKLEGFKEKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKKLIGEVSSGSGETFSYPDFLRMMLGKRSAILKMILMYEEKAREKEKPTGPPAKKAISELP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

PTMs:

Phosphorylated on serine residues.

細胞の位置付け:

Cytoplasm>Cytoskeleton. Cell projection>Ruffle membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Phagocytic cup.
Note: Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in T-lymphocytes and peripheral blood mononuclear cells.

参考文献

1). Stigmasterol attenuates inflammatory response of microglia via NF-κB and NLRP3 signaling by AMPK activation. Biomedicine & Pharmacotherapy, 2022 (PubMed: 35772378) [IF=6.9]

Application: IF/ICC    Species: Mouse    Sample: BV2 cells

Fig. 3. Effects of stigmasterol treatment on Aβ42 oligomers induced inflammatory response in BV2 cells. (A) Cell viability after Aβ42 oligomers treatment. (B-D) Concentration of TNFα, IL-1β and IL-10 after Aβ42 oligomers treatment. (E) Cell viability after stigmasterol treatment. (F, H) Concentration of TNFα, IL-1β and IL-10 after Aβ42 oligomers and stigmasterol treatment. (I) Representative images of CD86 expression in BV2 cells, measured by flow cytometry. (J) Quantification of CD86+ cells. (K) Representative images of immunofluorescence staining for Iba1 (red) in BV2 cells. Scale bar 40 µm. (L, M) Cell counts and quantification of Iba1 fluorescence intensity. Data were presented as the mean ± SEM from three independent experiments. One-way ANOVA with Tukey’s multiple comparison test revealed a difference between groups. *P 
2). Melatonin suppresses inflammation and blood‒brain barrier disruption in rats with vascular dementia possibly by activating the SIRT1/PGC-1α/PPARγ signaling pathway. Inflammopharmacology, 2023 (PubMed: 37017851) [IF=4.6]
3). Luteolin and Exercise Combination Therapy Ameliorates Amyloid-β1-42 Oligomers-Induced Cognitive Impairment in AD Mice by Mediating Neuroinflammation and Autophagy. Journal of Alzheimer's disease : JAD, 2023 (PubMed: 36710678) [IF=3.4]
4). Systematic analysis of critical genes and pathways identified a signature of neuropathic pain after spinal cord injury. European Journal of Neuroscience, 2022 (PubMed: 35560852) [IF=2.7]
5). Cinnamaldehyde Attenuates the Expression of IBA1 and GFAP to Inhibit Glial Cell Activation and Inflammation in the MPTP-Induced Acute Parkinson's Disease Model. Parkinson's disease, 2024 (PubMed: 39741957) [IF=2.1]

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