TRIM63 Antibody - #DF7187
(7)
(2)
製品説明
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7187, RRID:AB_2839139.
折りたたみ／展開
E3 ubiquitin-protein ligase TRIM63; FLJ32380; IRF; Iris RING finger protein; MURF 1; MURF-1; MuRF1; MURF2; Muscle specific ring finger protein 1; Muscle specific ring finger protein 2; Muscle-specific RING finger protein 1; OTTHUMP00000008701; RING finger protein 28; RNF 28; RNF28; SMRZ; Striated muscle RING zinc finger protein; TRI63_HUMAN; TRIM 63; Trim63; Tripartite motif containing 63; tripartite motif containing 63, E3 ubiquitin protein ligase; Tripartite motif containing protein 63; Tripartite motif-containing protein 63; Ubiquitin ligase TRIM63;
免疫原
A synthesized peptide derived from human TRIM63, corresponding to a region within N-terminal amino acids.
Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
- Q969Q1 TRI63_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDYKSSLIQDGNPMENLEKQLICPICLEMFTKPVVILPCQHNLCRKCANDIFQAANPYWTSRGSSVSMSGGRFRCPTCRHEVIMDRHGVYGLQRNLLVENIIDIYKQECSSRPLQKGSHPMCKEHEDEKINIYCLTCEVPTCSMCKVFGIHKACEVAPLQSVFQGQKTELNNCISMLVAGNDRVQTIITQLEDSRRVTKENSHQVKEELSQKFDTLYAILDEKKSELLQRITQEQEKKLSFIEALIQQYQEQLDKSTKLVETAIQSLDEPGGATFLLTAKQLIKSIVEASKGCQLGKTEQGFENMDFFTLDLEHIADALRAIDFGTDEEEEEFIEEEDQEEEESTEGKEEGHQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
研究背景
E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
Cytoplasm. Nucleus. Cytoplasm>Myofibril>Sarcomere>M line. Cytoplasm>Myofibril>Sarcomere>Z line.
Note: Colocalizes with TNNI3 in myocytes (By similarity). Localizes to the M- and Z-lines in skeletal muscle.
Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
The RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity (By similarity).
The B box-type zinc finger mediates homodimerization.
参考文献
Application: WB Species: Mouse Sample: GA tissues
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