製品: LRPAP1 Antibody
カタログ: DF7141
タンパク質の説明: Rabbit polyclonal antibody to LRPAP1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Dog, Chicken
分子量: 41kDa; 41kD(Calculated).
ユニプロット: P30533
RRID: AB_2839095

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(89%), Dog(100%), Chicken(92%)
クローナリティ:
Polyclonal
特異性:
LRPAP1 Antibody detects endogenous levels of total LRPAP1.
RRID:
AB_2839095
引用形式: Affinity Biosciences Cat# DF7141, RRID:AB_2839095.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

39 kDa receptor-associated protein; A2MRAP; A2RAP; Alpha 2 macroglobulin receptor associated protein; Alpha 2 MRAP; Alpha-2-macroglobulin receptor-associated protein; Alpha-2-MRAP; AMRP_HUMAN; HBP44; Lipoprotein receptor associated protein; Low density lipoprotein receptor related protein associated protein 1; Low density lipoprotein receptor-related protein-associated protein 1; Low density lipoprotein related protein associated protein 1 alpha 2 macroglobulin receptor associated protein; Low density lipoprotein related protein associated protein 1; low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1); Lrpap1; MGC138272; MRAP; MYP23; RAP;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
Low density lipoprotein receptor-related protein associated protein 1 also known as LRPAP1 or RAP is a chaperone protein which in humans is encoded by the LRPAP1 gene. LRPAP1 is involved with trafficking of certain members of the LDL receptor family including LRP1 and LRP2. It is a glycoprotein that binds to the alpha-2-macroglobulin receptor, as well as to other members of the low density lipoprotein receptor family. It acts to inhibit the binding of all know ligands for these receptors, and may prevent receptor aggregation and degradation in the endoplasmic reticulum, thereby acting as a molecular chaperone. It may be under the regulatory control of calmodulin, since it is able to bind calmodulin and be phosphorylated by calmodulin-dependent kinase II.
タンパク質配列:
MAPRRVRSFLRGLPALLLLLLFLGPWPAASHGGKYSREKNQPKPSPKRESGEEFRMEKLNQLWEKAQRLHLPPVRLAELHADLKIQERDELAWKKLKLDGLDEDGEKEARLIRNLNVILAKYGLDGKKDARQVTSNSLSGTQEDGLDDPRLEKLWHKAKTSGKFSGEELDKLWREFLHHKEKVHEYNVLLETLSRTEEIHENVISPSDLSDIKGSVLHSRHTELKEKLRSINQGLDRLRRVSHQGYSTEAEFEEPRVIDLWDLAQSANLTDKELEAFREELKHFEAKIEKHNHYQKQLEIAHEKLRHAESVGDGERVSRSREKHALLEGRTKELGYTVKKHLQDLSGRISRARHNEL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Chicken
92
Sheep
89
Pig
75
Zebrafish
67
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30533 基板として

Site PTM Type Enzyme
S45 Phosphorylation
S50 Phosphorylation
T134 O-Glycosylation
S135 Phosphorylation
S137 Phosphorylation
T141 Phosphorylation
K153 Ubiquitination
S165 Phosphorylation
Y186 Phosphorylation
S230 Phosphorylation
S242 Phosphorylation
S247 Phosphorylation
T248 Phosphorylation
N268 N-Glycosylation
K287 Acetylation
K304 Ubiquitination
S310 Phosphorylation
K332 Ubiquitination
Y336 Phosphorylation
T337 O-Glycosylation
S346 O-Glycosylation
R348 Methylation

研究背景

機能:

Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.

PTMs:

N-glycosylated.

細胞の位置付け:

Rough endoplasmic reticulum lumen. Endoplasmic reticulum-Golgi intermediate compartment lumen. Golgi apparatus>cis-Golgi network. Golgi apparatus lumen. Endosome lumen. Cell surface.
Note: May be associated with receptors at the cell surface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with the LRP1/alpha-2-macroglobulin receptor heavy and light chains; the interaction is transient and coincides with a reduction of ligand binding by the receptor. Interacts with LRP2/glycoprotein 330. Interacts with LRP1B; binding is followed by internalization and degradation. Interacts with LDLR. Interacts with SORL1. Interacts with LRP1; this interaction is followed by rapid internalization.

タンパク質ファミリー:

Belongs to the alpha-2-MRAP family.

研究領域

· Organismal Systems > Digestive system > Cholesterol metabolism.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.