IGFBP7 Antibody - #DF7131

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製品: IGFBP7 Antibody
カタログ: DF7131
タンパク質の説明: Rabbit polyclonal antibody to IGFBP7
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Bovine, Sheep, Rabbit, Dog
分子量: 29kDa(Observed); 29kD(Calculated).
ユニプロット: Q16270
RRID: AB_2839085

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#DF7131-BP

IGFBP7 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Sheep(100%), Rabbit(92%), Dog(92%)
クローナリティ:
Polyclonal
特異性:
IGFBP7 Antibody detects endogenous levels of total IGFBP7.
RRID:
AB_2839085
引用形式: Affinity Biosciences Cat# DF7131, RRID:AB_2839085.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AGM; Angiomodulin; FSTL2; IBP-7; IBP7; IBP7_HUMAN; IGF binding protein 7; IGF-binding protein 7; IGFBP rP1; IGFBP-7; IGFBP-rP1; IGFBP7; IGFBPRP1; Insulin like growth factor binding protein 7; Insulin-like growth factor-binding protein 7; MAC25; MAC25 protein; PGI2 stimulating factor; PGI2-stimulating factor; Prostacyclin stimulating factor; Prostacyclin-stimulating factor; PSF; RAMSVPS; TAF; Tumor-derived adhesion factor;

免疫原

免疫原:

A synthesized peptide derived from human IGFBP7, corresponding to a region within the internal amino acids.

Uniprot:

Q16270(IBP7_HUMAN) >>Visit HPA database.

遺伝子(ID):
IGFBP7(3490)
タンパク質の説明:
This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm.
タンパク質配列:
MERPSLRALLLGAAGLLLLLLPLSSSSSSDTCGPCEPASCPPLPPLGCLLGETRDACGCCPMCARGEGEPCGGGGAGRGYCAPGMECVKSRKRRKGKAGAAAGGPGVSGVCVCKSRYPVCGSDGTTYPSGCQLRAASQRAESRGEKAITQVSKGTCEQGPSIVTPPKDIWNVTGAQVYLSCEVIGIPTPVLIWNKVKRGHYGVQRTELLPGDRDNLAIQTRGGPEKHEVTGWVLVSPLSKEDAGEYECHASNSQGQASASAKITVVDALHEIPVKKGEGAEL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
92
Rabbit
92
Chicken
67
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.

PTMs:

N-glycosylated.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

参考文献

1). Interferon Regulatory Factor1 Deficiency Ameliorates Hemorrhagic Shock-Induced Kidney Injury Via IGFBP7/IGF1R Pathway. The Journal of surgical research, 2025 (PubMed: 41092604) [IF=1.8]
2). SM22α Loss Contributes to Apoptosis of Vascular Smooth Muscle Cells via Macrophage-Derived circRasGEF1B. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 33859778)

Application: WB    Species: Mice    Sample: VSMCs

Figure 2 SM22α loss contributes to interaction of VSMCs with macrophages via expression of VCAM-1. (a) Relative quantification for RAW264.7 cell migration induced by the CM from WT and Sm22α−/− VSMCs treated with (+) or without (-) Ang II. (b) The fluorescent intensity quantification of calcein-AM-labeled RAW264.7 cell adhesion to WT or Sm22α−/− VSMCs. (c) qRT-PCR of the mRNA of TNF-α, MCP-1, IL-6, and IL-1β in RAW264.7 cells stimulated by CM from WT or Sm22α−/− VSMCs. (d) The relative number of RAW264.7 cell adhesion to Ad-vector- or Ad-SM22α-infected Sm22α−/− VSMCs. (e) Relative quantification for RAW264.7 cell migration induced by the CM from Ad-vector- or Ad-SM22α-infected Sm22α−/− VSMCs. (f) qRT-PCR of the mRNA of TNF-α, MCP-1, IL-6, and IL-1β in RAW264.7 cells stimulated by CM from Ad-vector- or Ad-SM22α-infected Sm22α−/− VSMCs. (g) Western blot analysis of differentially expressed adhesion molecules in CM from WT or Sm22α−/− VSMCs. (h, i) The fluorescent intensity quantification of calcein-AM-labeled RAW264.7 cell adhesion to Sm22α−/− VSMCs transfected with si-VCAM-1 (h) or preincubated with IgG or VCAM-1 neutralizing antibody (i). Data are presented as mean ± SD of three independent experiments. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.

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