HMGA2 Antibody - #DF7126

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製品: HMGA2 Antibody
カタログ: DF7126
タンパク質の説明: Rabbit polyclonal antibody to HMGA2
アプリケーション: WB
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep
分子量: 12kDa; 12kD(Calculated).
ユニプロット: P52926
RRID: AB_2839080

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 100ul $280 在庫あり
 200ul $350 在庫あり

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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(88%), Bovine(100%), Sheep(100%)
クローナリティ:
Polyclonal
特異性:
HMGA2 Antibody detects endogenous levels of total HMGA2.
RRID:
AB_2839080
引用形式: Affinity Biosciences Cat# DF7126, RRID:AB_2839080.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

HMGA 2; BABL; High mobility group (nonhistone chromosomal) protein isoform I C; High mobility group (nonhistone chromosomal) protein isoform IC; High Mobility Group AT hook 2; High Mobility Group AT hook protein 2; High mobility group AT-hook protein 2; High mobility group protein HMGI C; High mobility group protein HMGI-C; High mobility group protein HMGIC; High mobility group protein I, isoform C; HMGA2; HMGA2_HUMAN; HMGI C; HMGIC; LIPO; Non histone chromosomal architectural protein HMGI C; Non histone chromosomal architectural protein HMGIC; Pygmy; STQTL9;

免疫原

免疫原:

A synthesized peptide derived from human HMGA2, corresponding to a region within C-terminal amino acids.

Uniprot:

P52926(HMGA2_HUMAN) >>Visit HPA database.

遺伝子(ID):
HMGA2(8091)
タンパク質の説明:
This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
タンパク質配列:
MSARGEGAGQPSTSAQGQPAAPAPQKRGRGRPRKQQQEPTGEPSPKRPRGRPKGSKNKSPSKAAQKKAEATGEKRPRGRPRKWPQQVVQKKPAQEETEETSSQESAEED

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Pig
88
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Plays a role in postnatal myogenesis, is involved in satellite cell activation (By similarity).

PTMs:

Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. Phosphorylated by NEK2 (By similarity).

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the HMGA family.

研究領域

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

参考文献

1). Long non-coding RNA LINC00460 promotes proliferation and inhibits apoptosis of cervical cancer cells by targeting microRNA-503-5p. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2020 (PubMed: 32740791) [IF=3.5]

Application: WB    Species: human    Sample: SiHa and HeLa cells

Fig. 4 | LINC00460 acts as a competing endogenous RNA of miR503-5p in SiHa and HeLa cells.d–h Expressions of AKT2,HMGA2 and SHOX2 in SiHa and HeLa cells by western blotting.**p<0.01 versus cells co-transfected with wild-type LINC00460 and miR-503-5p mimic. ##p<0.01 versus cells transfected with NC shRNA. WT wild-type LINC00460, MUT mutated LINC00460

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