Claudin 3 Antibody - #DF7115

Fig. 7. Biological effects of PS particles on the placenta and JEG-3 cells. (A) Representative images of H&E of placenta from indicated mice. scale bar = 2 mm. (B) The ratio of junction zone to the total placental area is shown. (C) Representative merged fluorescence images of cleaved caspase-3 in the placenta; scale bar = 100 μm. (D) The mean cleaved caspase-3 fluorescence intensity in the labyrinth zone of placenta is shown. (E) Representative merged fluorescence images of TUNEL in the labyrinth zone of placenta; scale bar = 100 μm. (F) The ratio of TUNEL-positive cells in the labyrinth zone of placenta is shown. (G) Representative merged fluorescence images of the fetal endothelium of placenta stained with cleaved CLDN3; scale bar = 100 μm. (H) Quantification of the mean CLDN3 fluorescence intensity in the fetal endothelium of placenta is shown. (I) TEM images of the gap between two cells in the fetal endothelium of placenta. White arrowhead represents the dilated gap, scale bar = 0.5 μm. (J) The total antioxidant capacity in the placenta is shown. (K) JEG-3 cells were exposed to PS particles at various doses for 24 h or 48 h, and cell viability was determined using the CCK-8 method. (L) Internalization of PS particles in JEG-3 cells was observed by confocal fluorescence microscopy. (M) Quantification of the mean PS particle fluorescence intensity in JEG-3 cells is shown. (N) JEG-3 cells were collected to measure DNA synthesis using an EdU proliferation assay; scale bar = 400 μm. (O) The ratio of EdU-positive JEG-3 cells is shown. (P) The cell cycle distributions of JEG-3 cells were measured using flow cytometry. (Q) JEG-3 cells were incubated with DCFH-DA for 20 min, and ROS levels were observed when JEG-3 cells were exposed to PS particles for 48 h; scale bar = 400 μm. (R) The quantification of the mean DCFH-DA fluorescence intensity in JEG-3 cells is shown. (S) JEG-3 cells were stained with annexin V-FITC/PI and analyzed by flow cytometry. (T) The protein expression of cleaved caspase-3 and BCL2 was measured by Western blotting and analyzed in JEG-3 cells. (U) Representative merged fluorescence images of JEG-3 cells stained with CLDN3; scale bar = 100 μm. (V) The protein expression of CLDN3 was measured by Western blotting and analyzed in JEG-3 cells. The data are presented as the means ± SD (*p < 0.05; **p < 0.01). In A, dashed lines delineate the decidua zone (D), junction zone (J) and labyrinth layer (L). In G, dashed lines delineate the fetal vessel endothelium.