Cytokeratin 7 Antibody - #DF7027

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製品: Cytokeratin 7 Antibody
カタログ: DF7027
タンパク質の説明: Rabbit polyclonal antibody to Cytokeratin 7
アプリケーション: WB IHC IF/ICC
Cited expt.: IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Sheep
分子量: 51kDa(Observed); 51kD(Calculated).
ユニプロット: P08729
RRID: AB_2838983

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#DF7027-BP

Cytokeratin 7 Peptide

1mg
関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
予測:
Bovine(88%), Sheep(%)
クローナリティ:
Polyclonal
特異性:
Cytokeratin 7 Antibody detects endogenous levels of total Cytokeratin 7.
RRID:
AB_2838983
引用形式: Affinity Biosciences Cat# DF7027, RRID:AB_2838983.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CK 7; CK-7; CK7; Cytokeratin 7; Cytokeratin-7; D15Wsu77e; K2C7; K2C7_HUMAN; K7; Keratin 7; Keratin 7, type II; Keratin type II cytoskeletal 7; Keratin, 55K type II cytoskeletal; Keratin, simple epithelial; Keratin, simple epithelial type I, K7; Keratin, type II cytoskeletal 7; Keratin-7; Krt2-7; KRT7; MGC11625; MGC129731; MGC3625; Sarcolectin; SCL; Type II mesothelial keratin K7; Type-II keratin Kb7;

免疫原

免疫原:

A synthesized peptide derived from human Cytokeratin 7, corresponding to a region within C-terminal amino acids.

Uniprot:

P08729(K2C7_HUMAN) >>Visit HPA database.

遺伝子(ID):
KRT7(3855)
発現特異性:
P08729 K2C7_HUMAN:

Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.

タンパク質の説明:
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.
タンパク質配列:
MSIHFSSPVFTSRSAAFSGRGAQVRLSSARPGGLGSSSLYGLGASRPRVAVRSAYGGPVGAGIREVTINQSLLAPLRLDADPSLQRVRQEESEQIKTLNNKFASFIDKVRFLEQQNKLLETKWTLLQEQKSAKSSRLPDIFEAQIAGLRGQLEALQVDGGRLEAELRSMQDVVEDFKNKYEDEINHRTAAENEFVVLKKDVDAAYMSKVELEAKVDALNDEINFLRTLNETELTELQSQISDTSVVLSMDNSRSLDLDGIIAEVKAQYEEMAKCSRAEAEAWYQTKFETLQAQAGKHGDDLRNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDARAKQEELEAALQRGKQDMARQLREYQELMSVKLALDIEIATYRKLLEGEESRLAGDGVGAVNISVMNSTGGSSSGGGIGLTLGGTMGSNALSFSSSAGPGLLKAYSIRTASASRRSARD

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
88
Sheep
88
Pig
0
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

PTMs:

Arg-20 is dimethylated, probably to asymmetric dimethylarginine.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.

タンパク質ファミリー:

Belongs to the intermediate filament family.

参考文献

1). Hepatic factor MANF drives hepatocytes reprogramming by detaining cytosolic CK19 in intrahepatic cholangiocarcinoma. Cell death and differentiation, 2024 (PubMed: 39972058) [IF=13.7]
2). Isolation, culture, and identification of ceruminous gland cells. Journal of Molecular Histology, 2022 (PubMed: 35113280) [IF=2.9]
3). Establishment and Characterization of a New Cell Line from Enzootic Nasal Adenocarcinoma in Goats: ENA-1. Veterinary sciences, 2024 (PubMed: 38922007) [IF=2.4]

Application: IF/ICC    Species: Mouse    Sample: ENA-1 cells

Figure 5 Immunofluorescence staining of ENA-1 cells for CK7 and CK18. ENA-1 cells expressed CK7 and CK18 in the cytoplasm, with CK18 most strongly expressed. (A) Negative control; (B) ENA-1 cells expressing CK7; and (C) ENA-1 cells strongly expressing CK18. (A–C), bar—100 μm.
4). The pathological characteristics of enzootic nasal adenocarcinoma in goats. Journal of veterinary research, 2025 (PubMed: 40144068) [IF=1.5]

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