製品: ELSPBP1 Antibody
カタログ: DF6874
タンパク質の説明: Rabbit polyclonal antibody to ELSPBP1
アプリケーション: WB IHC IF/ICC
反応性: Human
予測: Pig, Bovine, Horse, Sheep, Dog
分子量: 26kDa, 35 kDa; 26kD(Calculated).
ユニプロット: Q96BH3
RRID: AB_2838833

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Dog(90%)
クローナリティ:
Polyclonal
特異性:
ELSPBP1 Antibody detects endogenous levels of total ELSPBP1.
RRID:
AB_2838833
引用形式: Affinity Biosciences Cat# DF6874, RRID:AB_2838833.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

免疫原

免疫原:

A synthesized peptide derived from human ELSPBP1, corresponding to a region within C-terminal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
Q96BH3 ESPB1_HUMAN:

Detected in cauda epididymidal fluid and on sperm membrane (at protein level).

タンパク質の説明:
The protein encoded by this gene belongs to the sperm-coating protein family of epididymal origin. This protein and its canine homolog are the first known examples of proteins with four tandemly arranged fibronectin type 2 (Fn2) domains in the Fn2-module protein family. [provided by RefSeq, Jul 2008]
タンパク質配列:
MTRWSSYLLGWTTFLLYSYESSGGMHEECVFPFTYKGSVYFTCTHIHSLSPWCATRAVYNGQWKYCQSEDYPRCIFPFIYRGKAYNSCISQGSFLGSLWCSVTSVFDEKQQWKFCETNEYGGNSLRKPCIFPSIYRNNVVSDCMEDESNKLWCPTTENMDKDGKWSFCADTRISALVPGFPCHFPFNYKNKNYFNCTNEGSKENLVWCATSYNYDQDHTWVYC

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
90
Horse
88
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Binds to spermatozoa upon ejaculation and may play a role in sperm capacitation. Has phosphorylcholine-binding activity (By similarity).

PTMs:

N-glycosylated.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in cauda epididymidal fluid and on sperm membrane (at protein level).

タンパク質ファミリー:

Belongs to the seminal plasma protein family.

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