HSD17B2 Antibody - #DF6734

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製品: HSD17B2 Antibody
カタログ: DF6734
タンパク質の説明: Rabbit polyclonal antibody to HSD17B2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Dog
分子量: 42kDa; 43kD(Calculated).
ユニプロット: P37059
RRID: AB_2838696

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Dog(100%)
クローナリティ:
Polyclonal
特異性:
HSD17B2 Antibody detects endogenous levels of total HSD17B2.
RRID:
AB_2838696
引用形式: Affinity Biosciences Cat# DF6734, RRID:AB_2838696.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

17 beta HSD 2; 17-beta-HSD 2; 17-beta-hydroxysteroid dehydrogenase type 2; 20 alpha HSD; 20 alpha hydroxysteroid dehydrogenase; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; DHB2_HUMAN; E2DH; EDH17B2; Estradiol 17-beta-dehydrogenase 2; HSD17; Hsd17b2; Hydroxysteroid (17 beta) dehydrogenase 2; Microsomal 17 beta hydroxysteroid dehydrogenase; Microsomal 17-beta-hydroxysteroid dehydrogenase; SDR9C2; Short chain dehydrogenase/reductase family 9C, member 2; Testosterone 17 beta dehydrogenase; Testosterone 17-beta-dehydrogenase;

免疫原

免疫原:
Uniprot:

P37059(DHB2_HUMAN) >>Visit HPA database.

遺伝子(ID):
HSD17B2(3294)
タンパク質配列:
MSTFFSDTAWICLAVPTVLCGTVFCKYKKSSGQLWSWMVCLAGLCAVCLLILSPFWGLILFSVSCFLMYTYLSGQELLPVDQKAVLVTGGDCGLGHALCKYLDELGFTVFAGVLNENGPGAEELRRTCSPRLSVLQMDITKPVQIKDAYSKVAAMLQDRGLWAVINNAGVLGFPTDGELLLMTDYKQCMAVNFFGTVEVTKTFLPLLRKSKGRLVNVSSMGGGAPMERLASYGSSKAAVTMFSSVMRLELSKWGIKVASIQPGGFLTNIAGTSDKWEKLEKDILDHLPAEVQEDYGQDYILAQRNFLLLINSLASKDFSPVLRDIQHAILAKSPFAYYTPGKGAYLWICLAHYLPIGIYDYFAKRHFGQDKPMPRALRMPNYKKKAT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Xenopus
60
Pig
0
Horse
0
Bovine
0
Sheep
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P37059 基板として

Site PTM Type Enzyme
K141 Acetylation
S251 Phosphorylation
S259 Phosphorylation
T267 Phosphorylation
S273 Phosphorylation

研究背景

機能:

Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.

細胞の位置付け:

Membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

研究領域

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

参考文献

1). Single-cell RNA sequencing reveals that HSD17B2 in cancer-associated fibroblasts promotes the development and progression of castration-resistant prostate cancer. Cancer Letters (PubMed: 37244445) [IF=9.7]
2). The Curcumin Derivative, H10, Suppresses Hormone-Dependent Prostate Cancer by Inhibiting 17β-Hydroxysteroid Dehydrogenase Type 3. Frontiers in Pharmacology (PubMed: 32457626) [IF=5.6]

Application: WB    Species: Human    Sample: LC540 (17β-HSD3) cells

Figure 3 Effects of H10 on 17β-HSD3 in LC540 (17β-HSD3) cells. Effects of H10 on the production of (A) T and (B) P. (C) The expression of 17β-HSD3 mRNA. (D) The expression levels of the proteins in the T synthesis pathway were detected by western blotting following treatment with H10. The concentrations of H10 were 0.25, 0.5, and 1 μM, while the concentration of 3858858 (positive control) was 0.25 μM. (E) T produced from the microsomal fraction of rat testes containing 17β-HSD3. The data are reported as the mean ± sd (n = 3) of results obtained from individual experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 are considered to be significant, vs. the control.
3). Ormeloxifene, a selective estrogen receptor modulator, protects against pulmonary hypertension. European Journal of Pharmacology (PubMed: 36731722) [IF=5.0]

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