NR1I3 Antibody - #DF6725

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製品: NR1I3 Antibody
カタログ: DF6725
タンパク質の説明: Rabbit polyclonal antibody to NR1I3
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Horse
分子量: 39kDa(Observed); 40kD(Calculated).
ユニプロット: Q14994
RRID: AB_2838687

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#DF6725-BP

NR1I3 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Horse(100%)
クローナリティ:
Polyclonal
特異性:
NR1I3 Antibody detects endogenous levels of total NR1I3.
RRID:
AB_2838687
引用形式: Affinity Biosciences Cat# DF6725, RRID:AB_2838687.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CAR; CAR BETA; CAR1; CARA; Constitutive activator of retinoid response; constitutive active receptor; Constitutive active response; constitutive androstane nuclear receptor variant 2; constitutive androstane nuclear receptor variant 3; constitutive androstane nuclear receptor variant 4; constitutive androstane nuclear receptor variant 5; Constitutive androstane receptor; MB67; MGC97144; NR1I3; NR1I3_HUMAN; Nuclear receptor subfamily 1 group I member 3; orphan nuclear hormone receptor; Orphan nuclear receptor MB67; Orphan nuclear receptor NR1I3;

免疫原

免疫原:

A synthesized peptide derived from human NR1I3, corresponding to a region within the internal amino acids.

Uniprot:

Q14994(NR1I3_HUMAN) >>Visit HPA database.

遺伝子(ID):
NR1I3(9970)
発現特異性:
Q14994 NR1I3_HUMAN:

Predominantly expressed in liver.

タンパク質の説明:
This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene.
タンパク質配列:
MASREDELRNCVVCGDQATGYHFNALTCEGCKGFFRRTVSKSIGPTCPFAGSCEVSKTQRRHCPACRLQKCLDAGMRKDMILSAEALALRRAKQAQRRAQQTPVQLSKEQEELIRTLLGAHTRHMGTMFEQFVQFRPPAHLFIHHQPLPTLAPVLPLVTHFADINTFMVLQVIKFTKDLPVFRSLPIEDQISLLKGAAVEICHIVLNTTFCLQTQNFLCGPLRYTIEDGARVSPTVGFQVEFLELLFHFHGTLRKLQLQEPEYVLLAAMALFSPDRPGVTQRDEIDQLQEEMALTLQSYIKGQQRRPRDRFLYAKLLGLLAELRSINEAYGYQIQHIQGLSAMMPLLQEICS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element.

PTMs:

Phosphorylated at Thr-38 by PKC, dephosphorylation of Thr-38 is required for nuclear translocation and activation.

細胞の位置付け:

Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton.
Note: Recruited to the cytoplasm by DNAJC7.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Predominantly expressed in liver.

タンパク質ファミリー:

Composed by a short N-terminal domain followed by the DNA binding, hinge, and ligand binding/dimerization domains.

Belongs to the nuclear hormone receptor family. NR1 subfamily.

参考文献

1). Amelioration of Alcoholic Liver Disease by Activating PXR-Cytochrome P450s Axis with Blackberry Extract. Separations, 2022 [IF=2.6]

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