Claudin 5 Antibody - #AF0130

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製品: Claudin 5 Antibody
カタログ: AF0130
タンパク質の説明: Rabbit polyclonal antibody to Claudin 5
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Rabbit
分子量: 23kDa; 23kD(Calculated).
ユニプロット: O00501
RRID: AB_2833314

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MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(89%), Rabbit(89%)
クローナリティ:
Polyclonal
特異性:
Claudin 5 Antibody detects endogenous levels of total Claudin 5.
RRID:
AB_2833314
引用形式: Affinity Biosciences Cat# AF0130, RRID:AB_2833314.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Androgen withdrawal and apoptosis induced protein RVP1 like; AWAL; BEC 1; BEC1; Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome); Claudin-5; Claudin5; CLD5_HUMAN; CLDN 5; Cldn5; CPETR L1; CPETRL 1; CPETRL1; TMDVCF; TMVCF; Transmembrane protein deleted in VCFS; Transmembrane protein deleted in velocardiofacial syndrome;

免疫原

免疫原:
Uniprot:

O00501(CLD5_HUMAN) >>Visit HPA database.

遺伝子(ID):
CLDN5(7122)
タンパク質の説明:
claudin 5 Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO- 3. Interacts with MPDZ.
タンパク質配列:
MGSAALEILGLVLCLVGWGGLILACGLPMWQVTAFLDHNIVTAQTTWKGLWMSCVVQSTGHMQCKVYDSVLALSTEVQAARALTVSAVLLAFVALFVTLAGAQCTTCVAPGPAKARVALTGGVLYLFCGLLALVPLCWFANIVVREFYDPSVPVSQKYELGAALYIGWAATALLMVGGCLLCCGAWVCTGRPDLSFPVKYSAPRRPTATGDYDKKNYV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
89
Rabbit
89
Chicken
78
Xenopus
75
Horse
0
Sheep
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00501 基板として

Site PTM Type Enzyme
S86 Phosphorylation
Y148 Phosphorylation
S155 Phosphorylation
T207 Phosphorylation
T209 Phosphorylation
Y212 Phosphorylation
Y217 Phosphorylation

研究背景

機能:

Plays a major role in tight junction-specific obliteration of the intercellular space.

細胞の位置付け:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ (By similarity).

タンパク質ファミリー:

Belongs to the claudin family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

参考文献

1). Engagement of circular RNA HECW2 in the nonautophagic role of ATG5 implicated in the endothelial-mesenchymal transition. Autophagy (PubMed: 29260931) [IF=13.3]

Application: WB    Species: human    Sample: HBMECs

Figure 3|Role of ATG5 in LPS-induced EndoMT. Scale bar: 5 μm. Mice were administered LPS (0.83 mg/kg) once per day for 7 d. n=6 animals/group. *P<0.05,**P<0.01, ***P<0.001 versus saline group using the Mann-Whitney test. Transfection of HBMECs with siRNA ATG5 significantly inhibited the decreases in the TJP expression levels and the increases in the mesenchymal cell marker expression .
2). Activation of Sigma-1 Receptor Enhanced Pericyte Survival via the Interplay Between Apoptosis and Autophagy: Implications for Blood-Brain Barrier Integrity in Stroke. Translational Stroke Research (PubMed: 31290080) [IF=6.9]

Application: WB    Species: mouse    Sample: brain

Fig. 1| Knockdown of σ-1R aggravated cerebral ischemia-induced injury in pMCAO mice.h, i The decrease in TJP expression in the ipsilateral side of the brain observed at 24 h after pMCAO surgery was aggravated in σ-1R KO mice. TJP expression levels were determined via western blot analysis (h) and quantified through densitometric analysis.
3). Axl deficiency promotes the neuroinvasion of Japanese encephalitis virus by enhancing IL-1α production from pyroptotic macrophages. Journal of Virology (PubMed: 32611752) [IF=5.4]

Application: IF/ICC    Species: mouse    Sample: brains

Fig.9. |Effects of IL-1α alone on BBB permeability and tight junction integrity.(D) IF staining of claudin-5, occludin, and ZO-1 (red), CD31 (microvascular endothelial cell marker, green), and DAPI (nucleus, blue), scale bar=20 μm.

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