製品: MNAT1 Antibody
カタログ: DF6690
タンパク質の説明: Rabbit polyclonal antibody to MNAT1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 36kDa; 36kD(Calculated).
ユニプロット: P51948
RRID: AB_2838652

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(88%)
クローナリティ:
Polyclonal
特異性:
MNAT1 Antibody detects endogenous levels of total MNAT1.
RRID:
AB_2838652
引用形式: Affinity Biosciences Cat# DF6690, RRID:AB_2838652.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

MNAT 1; CAP35; CDK activating kinase assembly factor MAT1; CDK-activating kinase assembly factor MAT1; CDK7/cyclin H assembly factor; CDK7/cyclin-H assembly factor; Cyclin G1 interacting protein; Cyclin-G1-interacting protein; MAT1; MAT1_HUMAN; Menage a trois 1 (CAK assembly factor); Menage a trois; Menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis); MNAT CDK activating kinase assembly factor 1; Mnat1; p35; p36; RING finger protein 66; RING finger protein MAT1; RNF66; TFB3;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P51948 MAT1_HUMAN:

Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.

タンパク質の説明:
The protein encoded by this gene, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
タンパク質配列:
MDDQGCPRCKTTKYRNPSLKLMVNVCGHTLCESCVDLLFVRGAGNCPECGTPLRKSNFRVQLFEDPTVDKEVEIRKKVLKIYNKREEDFPSLREYNDFLEEVEEIVFNLTNNVDLDNTKKKMEIYQKENKDVIQKNKLKLTREQEELEEALEVERQENEQRRLFIQKEEQLQQILKRKNKQAFLDELESSDLPVALLLAQHKDRSTQLEMQLEKPKPVKPVTFSTGIKMGQHISLAPIHKLEEALYEYQPLQIETYGPHVPELEMLGRLGYLNHVRAASPQDLAGGYTSSLACHRALQDAFSGLFWQPS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
88
Zebrafish
88
Chicken
88
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51948 基板として

Site PTM Type Enzyme
M1 Acetylation
Y14 Phosphorylation
S18 Phosphorylation
T51 Phosphorylation
K70 Ubiquitination
K167 Ubiquitination
K176 Ubiquitination
K180 Ubiquitination
K202 Ubiquitination
S205 Phosphorylation
K219 Ubiquitination
T222 Phosphorylation
S224 Phosphorylation
T225 Phosphorylation
S234 Phosphorylation
S279 Phosphorylation

PTMs - P51948 酵素として

Substrate Site Source
P03372-1 (ESR1) S102 Uniprot
P03372-1 (ESR1) S104 Uniprot
P03372-1 (ESR1) S106 Uniprot

研究背景

機能:

Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.

サブユニット構造:

Associates primarily with CDK7 and cyclin H to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor.

研究領域

· Genetic Information Processing > Transcription > Basal transcription factors.

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

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