PADI4 Antibody - #DF6685

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製品: PADI4 Antibody
カタログ: DF6685
タンパク質の説明: Rabbit polyclonal antibody to PADI4
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Rabbit
分子量: 74kDa; 74kD(Calculated).
ユニプロット: Q9UM07
RRID: AB_2838647

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MS Report
HPLC Report
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説明書
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WB Handbook
IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Rabbit(82%)
クローナリティ:
Polyclonal
特異性:
PADI4 Antibody detects endogenous levels of total PADI4.
RRID:
AB_2838647
引用形式: Affinity Biosciences Cat# DF6685, RRID:AB_2838647.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

EC 3.5.3.15; HL 60 PAD; HL-60 PAD; PAD 4; PAD; PADI 4; PADI 5; PADI H protein; Padi4; PADI4_HUMAN; PADI5; PDI 4; PDI 5; PDI4; PDI5; Peptidyl arginine deiminase type IV; Peptidyl arginine deiminase type V; Peptidylarginine deiminase IV; Protein arginine deiminase; Protein arginine deiminase type 4; Protein arginine deiminase type IV; Protein-arginine deiminase type IV; Protein-arginine deiminase type-4;

免疫原

免疫原:

A synthesized peptide derived from human PADI4, corresponding to a region within N-terminal amino acids.

Uniprot:

Q9UM07(PADI4_HUMAN) >>Visit HPA database.

遺伝子(ID):
PADI4(23569)
発現特異性:
Q9UM07 PADI4_HUMAN:

Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.

タンパク質の説明:
This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response.
タンパク質配列:
MAQGTLIRVTPEQPTHAVCVLGTLTQLDICSSAPEDCTSFSINASPGVVVDIAHGPPAKKKSTGSSTWPLDPGVEVTLTMKVASGSTGDQKVQISYYGPKTPPVKALLYLTGVEISLCADITRTGKVKPTRAVKDQRTWTWGPCGQGAILLVNCDRDNLESSAMDCEDDEVLDSEDLQDMSLMTLSTKTPKDFFTNHTLVLHVARSEMDKVRVFQATRGKLSSKCSVVLGPKWPSHYLMVPGGKHNMDFYVEALAFPDTDFPGLITLTISLLDTSNLELPEAVVFQDSVVFRVAPWIMTPNTQPPQEVYACSIFENEDFLKSVTTLAMKAKCKLTICPEEENMDDQWMQDEMEIGYIQAPHKTLPVVFDSPRNRGLKEFPIKRVMGPDFGYVTRGPQTGGISGLDSFGNLEVSPPVTVRGKEYPLGRILFGDSCYPSNDSRQMHQALQDFLSAQQVQAPVKLYSDWLSVGHVDEFLSFVPAPDRKGFRLLLASPRSCYKLFQEQQNEGHGEALLFEGIKKKKQQKIKNILSNKTLREHNSFVERCIDWNRELLKRELGLAESDIIDIPQLFKLKEFSKAEAFFPNMVNMLVLGKHLGIPKPFGPVINGRCCLEEKVCSLLEPLGLQCTFINDFFTYHIRHGEVHCGTNVRRKPFSFKWWNMVP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
82
Horse
73
Bovine
55
Sheep
55
Pig
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.

PTMs:

Autocitrullination at Arg-372 and Arg-374 inactivates the enzyme.

細胞の位置付け:

Cytoplasm. Nucleus. Cytoplasmic granule.
Note: Cytoplasmic granules of eosinophils and neutrophils.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.

タンパク質ファミリー:

Belongs to the protein arginine deiminase family.

参考文献

1). Taohe Chengqi decoction inhibits PAD4-mediated neutrophil extracellular traps and mitigates acute lung injury induced by sepsis. Chinese journal of natural medicines, 2025 (PubMed: 41073060) [IF=4.0]
2). Silenced-C5ar1 improved multiple organ injury in sepsis rats via inhibiting neutrophil extracellular trap. Journal of molecular histology, 2024 (PubMed: 38165570) [IF=2.9]

Application: WB    Species: Rat    Sample:

Fig. 8 The expression levels of TLR2, TLR4, PAD4, and C5ar1 of peripheral blood neutrophils in CLP rats. a The protein bands of TLR2, TLR4, PAD4, and C5ar1; b–e the results of relative expression analysis. Data are presented as mean (standard deviation), n = 3. *P

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