VIP Antibody - #DF6627

Fig. 6 Pseudoephedrine + emodin up-regulated VIP/CAMP/PKA pathways and Inhibited NF-κB in LPS-induced acute lung injury in rats. A, D VIP, cAMP mRNA expression was determined using Real-time PCR analysis. B, C, E–H Western blot analysis was performed to detect VIP, cAMP, p-PKA, p-IκBα and p-P65 protein expression. All data are expressed as mean ± S.D. (n = 3). ##p < 0.01, ###p < 0.001 vs. control group. *p < 0.05, **p < 0.01, ***p < 0.001 vs. LPS alone group. +p < 0.05, ++p < 0.01, +++p < 0.001 vs. combined treatment group (5 + 20 mg/kg)

Fig. 9.| Effect of P.oil on the expression of VIP, cAMP, and PKA (n = 3–5). (A) Representative western blotting band. The expressions of VIP (B), cAMP (C) and PKA(D).

Figure 4 VIP is a direct target of miR-30c. (a) Wild type (WT) as well as mutated type (MUT) sequences of VIP for miR-30c target and dual luciferase report assay of VIP 3’-UTR wild type or MUT along with miR-30c. (b) The expression level of VIP was detected by qRT-PCR and Western Blot in the colon tissues of WT and miR-30c KO mice (n = 3). (c) Representative histopathology images in the colon slides of different groups’ mice by IHC staining for VIP. (Low Mag: Scale bars, 1000 μm; High Mag: Scale bars, 200 μm) (d) Statistical analysis of the average optical density values of positive signals in the colon slides of different groups’ mice (n = 5–6). (e) The expression level of miR-30c in the colon tissues of in the active ulcerative colitis patients and normal healthy control individuals (n = 4/group). (f) The expression level of VIP in the colon tissues of in the active ulcerative colitis patients and normal healthy control individuals (n = 4/group). Data are presented as the means ± SD and were analyzed with Student’s t-test.