MOK Antibody - #DF6618

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製品: MOK Antibody
カタログ: DF6618
タンパク質の説明: Rabbit polyclonal antibody to MOK
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 48kDa; 48kD(Calculated).
ユニプロット: Q9UQ07
RRID: AB_2838580

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説明書
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WB Handbook
IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
MOK Antibody detects endogenous levels of total MOK.
RRID:
AB_2838580
引用形式: Affinity Biosciences Cat# DF6618, RRID:AB_2838580.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Antigen recognized by autologous cytolytic T lymphocytes; EC 2.7.11.22; LE 9211 A antigen; MAPK/MAK/MRK overlapping kinase; MOK; MOK protein kinase; MOK_HUMAN; RAGE 1; RAGE; RAGE-1; RAGE1; Renal cell carcinoma antigen (MOK protein kinase); Renal tumor antigen 1; Renal tumor antigen; STK30;

免疫原

免疫原:

A synthesized peptide derived from human MOK, corresponding to a region within the internal amino acids.

Uniprot:

Q9UQ07(MOK_HUMAN) >>Visit HPA database.

遺伝子(ID):
MOK(5891)
発現特異性:
Q9UQ07 MOK_HUMAN:

Expressed in heart, brain, lung, kidney, and pancreas, and at very low levels in placenta, liver and skeletal muscle. Detected in retina.

タンパク質の説明:
This gene belongs to the MAP kinase superfamily. The gene was found to be regulated by caudal type transcription factor 2 (Cdx2) protein. The encoded protein, which is localized to epithelial cells in the intestinal crypt, may play a role in growth arrest and differentiation of cells of upper crypt and lower villus regions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Dec 2012]
タンパク質配列:
MKNYKAIGKIGEGTFSEVMKMQSLRDGNYYACKQMKQRFESIEQVNNLREIQALRRLNPHPNILMLHEVVFDRKSGSLALICELMDMNIYELIRGRRYPLSEKKIMHYMYQLCKSLDHIHRNGIFHRDVKPENILIKQDVLKLGDFGSCRSVYSKQPYTEYISTRWYRAPECLLTDGFYTYKMDLWSAGCVFYEIASLQPLFPGVNELDQISKIHDVIGTPAQKILTKFKQSRAMNFDFPFKKGSGIPLLTTNLSPQCLSLLHAMVAYDPDERIAAHQALQHPYFQEQRKTEKRALGSHRKAGFPEHPVAPEPLSNSCQISKEGRKQKQSLKQEEDRPKRRGPAYVMELPKLKLSGVVRLSSYSSPTLQSVLGSGTNGRVPVLRPLKCIPASKKTDPQKDLKPAPQQCRLPTIVRKGGR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Pig
75
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Able to phosphorylate several exogenous substrates and to undergo autophosphorylation. Negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner.

PTMs:

Autophosphorylated.

細胞の位置付け:

Cytoplasm. Cell projection>Cilium. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in heart, brain, lung, kidney, and pancreas, and at very low levels in placenta, liver and skeletal muscle. Detected in retina.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

参考文献

1). Class IIa HDAC Downregulation Contributes to Surgery-Induced Cognitive Impairment Through HMGB1-Mediated Inflammatory Response in the Hippocampi of Aged Mice. Journal of Inflammation Research, 2021 (PubMed: 34103963) [IF=4.5]

Application: WB    Species: Mice    Sample: hippocampus tissue

Figure 8 TLR4 and RAGE play a critical role in HMGB1-mediated neuroimmune activation. (A–C) Representative Western blots of TLR4, MyD88, and RAGE on postoperative day 1, 3, and 7, n = 4 per group. Each sample repeated the experiment 3 times. (D–F). Densitometric analysis of Western blot data from (A–C), n = 4 per group. #p < 0.05 versus the day-matched control group. *p < 0.05 versus the day-matched anesthesia group.

Application: WB    Species: mouse    Sample:

Figure 8| TLR4 and RAGE play a critical role in HMGB1-mediated neuroimmune activation. (A–C) Representative Western blots of TLR4, MyD88, and RAGE on postoperative day 1, 3, and 7, n = 4 per group. Each sample repeated the experiment 3 times.

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