製品: POLR2E Antibody
カタログ: DF6595
タンパク質の説明: Rabbit polyclonal antibody to POLR2E
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 24kDa; 25kD(Calculated).
ユニプロット: P19388
RRID: AB_2838557

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
POLR2E Antibody detects endogenous levels of total POLR2E.
RRID:
AB_2838557
引用形式: Affinity Biosciences Cat# DF6595, RRID:AB_2838557.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

and III subunit ABC1; and III subunit RPABC1; DNA directed RNA polymerase II 23 kda polypeptide; DNA directed RNA polymerase II 23 kDa polypeptide; DNA directed RNA polymerase II polypeptide E; DNA directed RNA polymerase II subunit E; DNA directed RNA polymerases I II and III subunit RPABC1; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; DNA-directed RNA polymerases I; hRPB25; hsRPB5; II; Polr2e; Polymerase (RNA) II (DNA directed) polypeptide E (25kD); Polymerase (RNA) II (DNA directed) polypeptide E, 25kDa; RNA polymerases I; RNA polymerases I, II, and III subunit ABC1; RPAB1_HUMAN; RPABC1; RPB5; RPB5 homolog; XAP4;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.
タンパク質配列:
MDDEEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQSGDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAPKYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVARYFGIKRGQVVKIIRPSETAGRYITYRLVQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P19388 基板として

Site PTM Type Enzyme
M1 Acetylation
R9 Methylation
K15 Ubiquitination
T29 Phosphorylation
T56 Phosphorylation
T59 Phosphorylation
K81 Ubiquitination
K85 Ubiquitination
K88 Ubiquitination
T111 Phosphorylation
K115 Ubiquitination
K186 Ubiquitination
K192 Ubiquitination

研究背景

機能:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity).

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively (By similarity). In RNA Pol II, this subunit is present in 2-fold molar excess over the other subunits. Interacts with URI1.

(Microbial infection) Interacts with HBV protein X.

タンパク質ファミリー:

Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family.

研究領域

· Genetic Information Processing > Transcription > RNA polymerase.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

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