Annexin V Antibody - #DF6580

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製品: Annexin V Antibody
カタログ: DF6580
タンパク質の説明: Rabbit polyclonal antibody to Annexin V
アプリケーション: WB IHC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 36kDa(Observed); 36kD(Calculated).
ユニプロット: P08758
RRID: AB_2838542

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Annexin V Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(100%), Chicken(90%), Xenopus(83%)
クローナリティ:
Polyclonal
特異性:
Annexin V Antibody detects endogenous levels of total Annexin V.
RRID:
AB_2838542
引用形式: Affinity Biosciences Cat# DF6580, RRID:AB_2838542.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Anchorin CII; Annexin 5; Annexin A5; Annexin V; Annexin-5; ANX A5; ANX5; ANXA5; ANXA5_HUMAN; Calphobindin I; CBP-I; Endonexin II; ENX2; Lipocortin V; PAP I; PAP-I; Placental anticoagulant protein 4; Placental anticoagulant protein I; PLACENTAL PROTEIN 4; Pp4; Thromboplastin inhibitor; VAC-alpha; Vascular anticoagulant-alpha;

免疫原

免疫原:

A synthesized peptide derived from human Annexin V, corresponding to a region within C-terminal amino acids.

Uniprot:

P08758(ANXA5_HUMAN) >>Visit HPA database.

遺伝子(ID):
ANXA5(308)
タンパク質の説明:
Annexin V, also known as PAP-1 or Lipocortin V, is a ~30 kDa protein that binds to phospholipids in a calcium-dependent manner (1). All annexins contain a putative PKC binding site, but only annexin V has been identified as an inhibitor of this pathway (2). It may also signal, by direct interaction with VEGFR2 receptor, in the regulation of vascular endothelial cell proliferation (3). Annexin V preferentially binds phosphatidylserine, in competition with prothrombin, leading to inhibition of blood coagulation at sites of injury preceding contact between lipid components and coagulation factors that initiate thrombosis (4-6). The ability of Annexin V to bind specifically and robustly to hosphatidylserine makes it an attractive reagent in detecting apoptotic cells (7). Annexin V is inducible by glucocorticoids and can be phosphorylated by tyrosine and serine/threonine kinases (8). It isthought to blockproduction of mediators of inflammation, such as prostaglandins and leukotrienes by inhibiting the release of arachidonic acid from membranes by phospholipase A2 (8).
タンパク質配列:
MAQVLRGTVTDFPGFDERADAETLRKAMKGLGTDEESILTLLTSRSNAQRQEISAAFKTLFGRDLLDDLKSELTGKFEKLIVALMKPSRLYDAYELKHALKGAGTNEKVLTEIIASRTPEELRAIKQVYEEEYGSSLEDDVVGDTSGYYQRMLVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFITIFGTRSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAYLAETLYYAMKGAGTDDHTLIRVMVSRSEIDLFNIRKEFRKNFATSLYSMIKGDTSGDYKKALLLLCGEDD

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Horse
92
Sheep
92
Rabbit
92
Chicken
90
Xenopus
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

PTMs:

S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

タンパク質ファミリー:

The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Belongs to the annexin family.

参考文献

1). Sonodynamic therapy of glioblastoma mediated by platelets with ultrasound-triggered drug release. Drug delivery, 2023 (PubMed: 37264811) [IF=6.5]

Application: IHC    Species: Mouse    Sample:

Figure 7. Sonotoxicity of GBM mediated by sono-activated IOPD-Ce6@Plt. IHC staining of annexin-V (A) (marker of apoptosis) and γH2AX (B), p-ATM (C) (markers of DNA damage) and H&E staining (D) in GBM tumors at the end of therapy. Treatment protocol is shown in Figure 6(A).
2). Human annexin A5 promotes glioma progression by targeting the MAPK/CD44 pathway. Frontiers in oncology, 2025 (PubMed: 40607003) [IF=3.5]

Application: WB    Species: human    Sample: U251 and SHG44 cells

Figure 8. Down-regulation of ANXA5 inhibited proliferation, migration, and invasion, and promoted cell apoptosis in the U251 and SHG44 cells. The mRNA (A) and protein (B) expression levels of ANXA5 were determined by qRT-PCR and western blot, respectively. (C-D) Transfection efficiency of ANXA5 knock-downed shRNA in U251 and SHG44 cells was determined by qRT-PCR and western blot. (E–I) The effects of ANXA5 down-regulation on U251 and SHG44 cell proliferation, migration, invasion, cell apoptosis, and cell cycle were determined by CCK-8, colony formation, Transwell assays (Scale bar, 100 μm), and flow cytometry analysis, respectively. (J) The protein expression of Bax, Bcl-2, Caspase-3, MMP-2, MMP-9, Vimentin, E-cadherin, p21, Cyclin A, CDK1, cyclin B, and CDK2 was measured by western blot. Data were expressed as mean ± SD. ▲ P 
3). 膜联蛋白A5在胰腺癌中的预后意义及机制的生物信息学分析. 中国普通外科杂志, 2022

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