CHI3L1 Antibody - #DF6577

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製品: CHI3L1 Antibody
カタログ: DF6577
タンパク質の説明: Rabbit polyclonal antibody to CHI3L1
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Rabbit, Dog
分子量: 43kDa; 43kD(Calculated).
ユニプロット: P36222
RRID: AB_2838539

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(88%), Bovine(100%), Rabbit(88%), Dog(86%)
クローナリティ:
Polyclonal
特異性:
CHI3L1 Antibody detects endogenous levels of total CHI3L1.
RRID:
AB_2838539
引用形式: Affinity Biosciences Cat# DF6577, RRID:AB_2838539.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

39 kDa synovial protein; ASRT7; Cartilage glycoprotein 39; CGP-39; CGP39; CH3L1_HUMAN; CHI3L1; chitinase 3 like 1 (cartilage glycoprotein 39); chitinase 3 like 1; Chitinase 3 like protein 1 precursor; chitinase; Chitinase-3-like protein 1; Chondrocyte protein YKL40; GP 39; GP-39; GP39; HC gp39; HCGP 3P; hCGP-39; HCgp39; YKL 40; YKL-40; YKL40; YYL 40;

免疫原

免疫原:

A synthesized peptide derived from human CHI3L1, corresponding to a region within the internal amino acids.

Uniprot:

P36222(CH3L1_HUMAN) >>Visit HPA database.

遺伝子(ID):
CHI3L1(1116)
発現特異性:
P36222 CH3L1_HUMAN:

Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.

タンパク質の説明:
Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]
タンパク質配列:
MGVKASQTGFVVLVLLQCCSAYKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Pig
88
Rabbit
88
Dog
86
Sheep
75
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.

PTMs:

Glycosylated.

細胞の位置付け:

Secreted>Extracellular space. Cytoplasm. Cytoplasm>Perinuclear region. Endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.

タンパク質ファミリー:

Belongs to the glycosyl hydrolase 18 family.

参考文献

1). Chitinase 3-like 1 overexpression aggravates hypoxia-reoxygenation injury in IEC-6 cells by inhibiting the PI3K/AKT signalling pathway. Experimental physiology, 2024 (PubMed: 39480684) [IF=2.6]

Application: WB    Species: Rat    Sample: IEC-6 cells

FIGURE 5 CHI3L1 level in different groups detected using western blotting. CHI3L1-si-1 and CHI3L1-si-2 were transfected into IEC-6 cells to knock down CHI3L1 and NC-si was transfected as a control. CHI3L1-OE was transfected into IEC-6 cells to overexpress CHI3L1. After transfection, IEC-6 cells were cultured under hypoxia for 2 h and reoxygenated for 6 h (H/R). Cells cultured under normoxia were used as a control. Three data points were overlaid on the bar graph. For all groups, n = 3.
2). YKL-40 Knockdown Decreases Oxidative Stress Damage in Ovarian Granulosa Cells. Genetic testing and molecular biomarkers, 2024 (PubMed: 38634621) [IF=1.1]

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