Cytochrome P450 11A1 Antibody - #DF6569

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製品: Cytochrome P450 11A1 Antibody
カタログ: DF6569
タンパク質の説明: Rabbit polyclonal antibody to Cytochrome P450 11A1
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat, Pig
分子量: 60kDa(Observed); 60kD(Calculated).
ユニプロット: P05108
RRID: AB_2838531

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Pig
クローナリティ:
Polyclonal
特異性:
Cytochrome P450 11A1 Antibody detects endogenous levels of total Cytochrome P450 11A1.
RRID:
AB_2838531
引用形式: Affinity Biosciences Cat# DF6569, RRID:AB_2838531.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Cholesterol 20 22 desmolase; Cholesterol desmolase; Cholesterol monooxygenase (side chain cleaving); Cholesterol side chain cleavage enzyme; Cholesterol side chain cleavage enzyme mitochondrial; Cholesterol side-chain cleavage enzyme; CP11A_HUMAN; CYP11A; CYP11A1; CYPXIA1; Cytochrome P450 11A1; Cytochrome P450 11A1 mitochondrial; Cytochrome P450 family 11 subfamily A polypeptide 1; Cytochrome P450 subfamily XIA; Cytochrome P450(scc); Cytochrome P450C11A1; mitochondrial; P450SCC; Steroid 20 22 lyase;

免疫原

免疫原:

A synthesized peptide derived from human Cytochrome P450 11A1, corresponding to a region within C-terminal amino acids.

Uniprot:

P05108(CP11A_HUMAN) >>Visit HPA database.

遺伝子(ID):
CYP11A1(1583)
タンパク質の説明:
The formation of biologically active steroid hormones is significantly influenced by the motrochondrial cytochrome P450 enzyme, chrolesterol side-chain cleavage. CYP11A1 functions in catalyzing the first step of steroid biosynthesis under modulation of cAMP.It ia also known as cytochrome P450C11A1, cytochrome P450scc and cytochrome P450, subfamily XIA. Expression of CYP11A1 has been a useful tool for monitoring the differentiation state of cells from varius endocrine tissues. Identification of CYP11A1 expression by 13363-1-AP detected a ~45kd band in human placenta, with respect to the reported band at 49-52kd by Roby, et al and Durkee, et al.
タンパク質配列:
MLAKGLPPRSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAAWKKDRVALNQEVMAPEATKNFLPLLDAVSRDFVSVLHRRIKKAGSGNYSGDISDDLFRFAFESITNVIFGERQGMLEEVVNPEAQRFIDAIYQMFHTSVPMLNLPPDLFRLFRTKTWKDHVAAWDVIFSKADIYTQNFYWELRQKGSVHHDYRGILYRLLGDSKMSFEDIKANVTEMLAGGVDTTSMTLQWHLYEMARNLKVQDMLRAEVLAARHQAQGDMATMLQLVPLLKASIKETLRLHPISVTLQRYLVNDLVLRDYMIPAKTLVQVAIYALGREPTFFFDPENFDPTRWLSKDKNITYFRNLGFGWGVRQCLGRRIAELEMTIFLINMLENFRVEIQHLSDVGTTFNLILMPEKPISFTFWPFNQEATQQ

研究背景

機能:

A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).

細胞の位置付け:

Mitochondrion inner membrane>Peripheral membrane protein.
Note: Localizes to the matrix side of the mitochondrion inner membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the cytochrome P450 family.

研究領域

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

参考文献

1). Interleukin 17A deficiency alleviates fluoride-induced testicular injury by inhibiting the immune response and apoptosis. Chemosphere, 2021 (PubMed: 33297146) [IF=8.1]

Application: WB    Species: Mice    Sample: testes

Fig. 5. IL-17A mediates the effect of excessive F on the testosterone level in serum and the expression of testosterone secretion-related proteins in testis (A) The content of testosterone in serum of mice (n ¼ 13. Three times repetition). (B) The expression of testosterone secretion-related proteins in testis (n ¼ 13. Three times repetition). Statistical analysis was carried out using one-way ANOVA with Dunnett’s test. Error bars denote the mean ± s.e.m. *P < 0.05, **P < 0.01.
2). The Curcumin Derivative, H10, Suppresses Hormone-Dependent Prostate Cancer by Inhibiting 17β-Hydroxysteroid Dehydrogenase Type 3. Frontiers in Pharmacology, 2020 (PubMed: 32457626) [IF=5.6]

Application: WB    Species: mouse    Sample: LC540 (17b-HSD3) cells

FIGURE 3 | Effects of H10 on 17b-HSD3 in LC540 (17b-HSD3) cells. Effects of H10 on the production of (A) T and (B) P. (C) The expression of 17b-HSD3 mRNA.(D) The expression levels of the proteins in the T synthesis pathway were detected by western blotting following treatment with H10. The concentrations of H10 were 0.25, 0.5, and 1 mM, while the concentration of 3858858 (positive control) was 0.25 mM.
3). A novel method to improve sow reproductive performance: Combination of pre-weaning immunization against inhibin and post-insemination hCG treatment. Journal of Integrative Agriculture, 2020 [IF=4.6]
4). Suppression of Notch Signaling Stimulates Progesterone Synthesis by Enhancing the Expression of NR5A2 and NR2F2 in Porcine Granulosa Cells. Genes, 2020 (PubMed: 31978970) [IF=3.5]

Application: WB    Species: porcine    Sample: pGCs

Figure 4. |Effects of NR5A2 knockdown on progesterone biosynthesis. (D,E) protein expression of NR5A2, StAR, Cyp11a1, and HSD3B. Primary pGCs were transfected with NR5A2 siRNA (siNR5A2) or scramble siRNA (siNC). Six hours later, the medium was changed to fresh medium with DMSO or DAPT, followed by further culture for 48 h. * means significant difference at p < 0.05.
5). Effects of LPS on the accumulation of lipid droplets, proliferation, and steroidogenesis in goat luteinized granulosa cells. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, 2019 (PubMed: 30934154) [IF=3.2]

Application: WB    Species: goat    Sample: LGCs

FIGURE 5| Effect of LPS on the key regulators during steroidogenesis in goat LGCs. Cells were cultured as in the figure.A, Cells were processed to analyze the expressions of STAR, P450scc, 3β‐HSD,and CYP19A1 protein by Western blot analysis. α‐Tubulin was used as an internal control.
6). Quercetin Alleviated H2O2-Induced Apoptosis and Steroidogenic Impairment in Goat Luteinized Granulosa Cells. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, 2020 (PubMed: 32410385) [IF=3.2]

Application: WB    Species: goat    Sample: granulosa cells

FIGURE 1 Identification of luteinized granulosa cells (GCs). A, The production of progesterone (P4) in goat GCs was enhanced in the forskolin group, compared with the fetal bovine serum (FBS) group. B‐G, The messenger RNA (mRNA) expressions of CYP19A1, CCND2, and PCNA decreased in the forskolin group, whereas there was an increase in the mRNA expressions of PTGFR, HSD3B, and StAR. The relative expression levels were normalized to GAPDH. H‐J, The expression of HSD3B and P450scc proteins increased in the forskolin group. Data are presented as mean ± SEM of three independent experiments. Different letters indicate significant differences (P < .05)
7). Effects of high-intensity interval training on adipose tissue lipolysis, inflammation, and metabolomics in aged rats. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2020 (PubMed: 32006095) [IF=2.9]

Application: WB    Species: rat    Sample: fat

Fig. 4 |Metabolic pathway enrichment analysis and lipolysis metabolismrelated protein and mRNA expression. Correlation analysis of 9 metabolites (a); metabolic pathway analysis in MICT versus SED (b);mRNA levels of PPAR-γ, HSL, ATGL, and TNF-α in adipose tissues (c);correlation analysis of 6 metabolites (d); metabolic pathway analysis in HIIT versus SED (e); and contents of PPAR-γ, HSL, ATGL, TNF-α, and P450scc protein (f).
8). Dual role of NR4A1 in porcine ovarian granulosa cell differentiation and granulosa-lutein cell regression in vitro. THERIOGENOLOGY, 2023 (PubMed: 36634443) [IF=2.4]

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