DCN Antibody - #DF6543

Figure 1. Enrichment analysis and expression profiles of differentially expressed genes (DEGs). (a) Functional map for downregulated DEGs enriched for GO terms. (b) Statistical analysis of the top 30 enriched KEGG pathways during PEDV infection. The x-axis indicates the factor of enrichment, whereas the y-axis indicates the KEGG channel. The dot color indicates the q value. The dot size corresponds to the number of DEGs mapped to the reference category. (c and d) Hierarchical clustering of extracellular matrix-constituent (c) and -regulated (d) DEGs. Rows indicate hierarchical clustering in accordance with average linkage based on Pearson correlation coefficients with the measured distance. Expression was visualized using a gradient color scheme; the scale from the minimal to maximal abundance fell in the range of −3.0 to 3.0. Blue represents low expression, whereas red represents high expression. (e) The total protein collected from the small intestine of PEDV-infected piglets (48 h post-infection) was prepared, and the expression levels of ECM-associated proteins (i.e., COL4A1, LAMB2, ITG6, and DCN) and PEDV N protein were determined by western blotting. The data shown are means ± SDs from triplicate experiments. *P < 0.05, **P < 0.01