OLR1 Antibody - #DF6522

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製品: OLR1 Antibody
カタログ: DF6522
タンパク質の説明: Rabbit polyclonal antibody to OLR1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 30~55kDa; 31kD(Calculated).
ユニプロット: P78380
RRID: AB_2838484

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
OLR1 Antibody detects endogenous levels of total OLR1.
RRID:
AB_2838484
引用形式: Affinity Biosciences Cat# DF6522, RRID:AB_2838484.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C-type lectin domain family 8 member A; CLEC8A; hLOX 1; hLOX-1; Lectin like oxidized LDL receptor 1; Lectin like oxLDL receptor 1; Lectin type oxidized LDL receptor 1; Lectin-like oxidized LDL receptor 1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1; low density lipoprotein oxidized, receptor 1; LOX-1; LOXIN; Olr1; OLR1_HUMAN; Ox LDL receptor 1; Ox-LDL receptor 1; Oxidised low density lipoprotein (lectin like) receptor 1; Oxidized low density lipoprotein receptor 1; Oxidized low density lipoprotein receptor 1 soluble form; Oxidized low-density lipoprotein receptor 1; SCARE1; Scavenger receptor class E, member 1; SLOX1; soluble form; SR-EI;

免疫原

免疫原:
Uniprot:

P78380(OLR1_HUMAN) >>Visit HPA database.

遺伝子(ID):
OLR1(4973)
発現特異性:
P78380 OLR1_HUMAN:

Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level.

タンパク質の説明:
This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.
タンパク質配列:
MTFDDLKIQTVKDQPDEKSNGKKAKGLQFLYSPWWCLAAATLGVLCLGLVVTIMVLGMQLSQVSDLLTQEQANLTHQKKKLEGQISARQQAEEASQESENELKEMIETLARKLNEKSKEQMELHHQNLNLQETLKRVANCSAPCPQDWIWHGENCYLFSSGSFNWEKSQEKCLSLDAKLLKINSTADLDFIQQAISYSSFPFWMGLSRRNPSYPWLWEDGSPLMPHLFRVRGAVSQTYPSGTCAYIQRGAVYAENCILAAFSICQKKANLRAQ

PTMs - P78380 基板として

Site PTM Type Enzyme
K7 Ubiquitination
K12 Ubiquitination
S19 Phosphorylation
K80 Ubiquitination
S95 Phosphorylation
S98 Phosphorylation
K112 Ubiquitination
K118 Ubiquitination
N139 N-Glycosylation
K171 Ubiquitination
K178 Ubiquitination

研究背景

機能:

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.

PTMs:

The intrachain disulfide-bonds prevent N-glycosylation at some sites.

N-glycosylated.

細胞の位置付け:

Cell membrane>Lipid-anchor. Cell membrane>Single-pass type II membrane protein. Membrane raft. Secreted.
Note: A secreted form also exists. Localization to membrane rafts requires palmitoylation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level.

サブユニット構造:

Homodimer; disulfide-linked. May form a hexamer composed of 3 homodimers. Interacts with HSP70.

タンパク質ファミリー:

The cytoplasmic region is required for subcellular sorting on the cell surface.

The C-type lectin domain mediates the recognition and binding of oxLDL.

研究領域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Organismal Systems > Endocrine system > PPAR signaling pathway.

参考文献

1). MiR-144-5p limits experimental abdominal aortic aneurysm formation by mitigating M1 macrophage-associated inflammation: Suppression of TLR2 and OLR1. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (PubMed: 32278833) [IF=5.0]
2). LOX-1 attenuates high glucose-induced autophagy via AMPK/HNF4α signaling in HLSECs. Heliyon (PubMed: 36590506) [IF=4.0]

Application: WB    Species: Human    Sample: HLSECs

Figure 1 The effect of high glucose on the expression levels of LOX-1, Beclin-1, and LC3II in HLSECs transfected by recombinant LV-LOX-1. HLSECs were treated with high glucose (HG), HG + recombinant lentivirus LOX-1 (LV-LOX-1), and HG + control lentiviral cells (LV-CON) for 72 h. (A–C) The mRNA expression levels of LOX-1, Beclin-1, and LC3II in HLSECs within 72 h after treatment with HG (25 mM), LV-LOX-1, and LV-CON. (D) The protein expression levels of each group were detected using western blotting (see original blots in Supplemental Figure 1D). (E–G) Western blotting analysis of the protein expression levels of LOX-1, Beclin-1, and LC3II in HLSECs within 72 h after treatment with HG and/or LV-LOX-1 and LV-CON. Western blot and qRT-PCR analyses were used to normalize the levels of GAPDH and β-actin (n = 3 independent experiments). All results are expressed as mean ± SD. ∗∗∗P < 0.001 versus the NC group; ###P < 0.001 versus HG + LV-CON group.

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