IFNB1 Antibody - #DF6471

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製品: IFNB1 Antibody
カタログ: DF6471
タンパク質の説明: Rabbit polyclonal antibody to IFNB1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
分子量: 22kDa; 22kD(Calculated).
ユニプロット: P01574
RRID: AB_2838433

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
IFNB1 Antibody detects endogenous levels of total IFNB1.
RRID:
AB_2838433
引用形式: Affinity Biosciences Cat# DF6471, RRID:AB_2838433.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

beta-interferon; Fibroblast interferon; IFB; IFF; IFN beta; IFN-beta; IFNB 1; IFNB; IFNB_HUMAN; IFNB1; Interferon beta 1 fibroblast; Interferon beta; Interferon beta precursor; MGC96956;

免疫原

免疫原:
Uniprot:

P01574(IFNB_HUMAN) >>Visit HPA database.

遺伝子(ID):
IFNB1(3456)
タンパク質の説明:
The genes encoding type I interferons (IFNs), which include 14 IFN- genes, one IFN-∫ gene, one IFN-w (also known as IFN- II1) gene, and a number of IFN-w pseudogenes, are clustered on human chromosome 9. Interferons- and -∫ are cytokines that are widely known to induce potent antiviral activity. IFN- and -∫ exert a variety of other biological effects, including antitumor and immunomodulatory activities and are increasingly used clinically to treat a range of malignancies, myelodysplasias and autoimmune diseases. IFN-w is antigenically different from human IFN- , IFN-∫ or IFN- , but is a component of natural mixtures of IFN species produced by virus-induced leukocytes or Burkitt's lymphoma cells. The type I interferon receptor (IFN- R) interacts with IFN- , IFN-∫ and IFN-w, and seems to be a multisubunit receptor.
タンパク質配列:
MTNKCLLQIALLLCFSTTALSMSYNLLGFLQRSSNFQCQKLLWQLNGRLEYCLKDRMNFDIPEEIKQLQQFQKEDAALTIYEMLQNIFAIFRQDSSSTGWNETIVENLLANVYHQINHLKTVLEEKLEKEDFTRGKLMSSLHLKRYYGRILHYLKAKEYSHCAWTIVRVEILRNFYFINRLTGYLRN

PTMs - P01574 基板として

Site PTM Type Enzyme
S140 Phosphorylation

研究背景

機能:

Has antiviral, antibacterial and anticancer activities.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Monomer. Signals mostly via binding to a IFNAR1-IFNAR2 heterodimeric receptor, but can also function with IFNAR1 alone and independently of Jak-STAT pathways.

タンパク質ファミリー:

Belongs to the alpha/beta interferon family.

研究領域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

参考文献

1). Hyperphosphorylated tau mediates neuronal death by inducing necroptosis and inflammation in Alzheimer’s disease. Journal of Neuroinflammation (PubMed: 35971179) [IF=9.3]

Application: WB    Species: Mouse    Sample: HT22 cells

Fig. 2Hyperphosphorylated tau induces cytokine upregulation. A Differentially expressed genes (DEGs) between HT22 cells transfected with vector or TauP301S for 48 h. B Analysis of DEGs in the TauP301S and Tau groups according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. C Heat map of cytokine genes differentially expressed in HT22 cells transfected with vector or Tau or TauP301S following treatment with DMSO or Nec-1 (30 μM); low expression is shown in blue and high expression in red. D mRNAs from HT22 cells transfected with vector or TauP301S following treatment with DMSO or Nec-1 (30 μM) were extracted and quantified to determine indicated cytokine levels by qPCR. E Cytokine levels in HT22 cells transfected with vector or TauP301S following treatment with DMSO or Nec-1 (30 μM) were analyzed by western blotting with the indicated antibodies. F ROS levels in HT22 cells transfected with vector or TauP301S. G Chemotaxis of pTau-induced cytokines on BV2 cells was analyzed by transwell assays, Scale bars, 100 μm. Data are presented as the mean ± standard error of the mean (SEM) of three experiments, and statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test in D and two-tailed unpaired t test in F, G
2). Tongmai granules improve rat hippocampal injury by regulating TLR4/MyD88/AP-1 signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY (PubMed: 34838942) [IF=5.4]
3). Effects of acute endurance exercise and exhaustive exercise on innate immune signals induced by mtDNA. European Journal of Inflammation [IF=0.7]

Application: WB    Species: Mouse    Sample: liver

Figure 3. Effects of acute exercise of different intensity on TLR9 and cGAS/STING signaling in liver, spleen, and leukocytes. (a, b, c) Effects of acute exercise of different intensity on mRNA expression of TLR9 and cGAS/STING signaling related molecules in liver (a), spleen (b), and leukocytes (c) (n = 6). Expression was normalized to 18S RNA. (d, e, f) Effects of acute exercise of different intensity on protein expression of TLR9 and cGAS/STING signaling related molecules in liver (d), spleen (e), and leukocytes (f) (n = 6). Expression was normalized to 18S RNA. (g, h) Effects of different intensity of acute exercise on plasma levels of IFN-β (g) and IL-6 (h) (n = 6). The density of each band was normalized to β-actin.
4). Mesenchymal Stem Cells Carrying IFN-α and IFN-β Overexpression Genes Inhibit Non-Small Cell Lung Cancer via the JAK/STAT Pathway. Journal of Evolutionary Biochemistry and Physiology [IF=0.6]

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